Anti-EGR1 Rabbit Polyclonal Antibody for WB and ELISA - P18146

Core Product Specifications and Parameters

Biological Background: EGR1 Function and Localization

• EGR1 is a transcriptional regulator that recognizes and binds to the EGR-site (5'-GCG(T/G)GGGCG-3') in target gene promoters (By similarity). It plays a role in responses to growth factors, DNA damage, and ischemia (PubMed:20121949). Related references: PMID:20121949
• It binds double-stranded target DNA independently of cytosine methylation status (PubMed:25258363, PubMed:25999311). Related references: PMID:25258363 PMID:25999311
• Activates tumor suppressor pathways by inducing p53/TP53 and TGFB1 expression, contributing to tumor prevention.
• Required for normal mitotic progression and hepatocyte proliferation; mediates ischemia and hypoxia responses by regulating inflammatory genes such as IL1B and CXCL2.
• Regulates luteinizing hormone biosynthesis in the pituitary and circadian clock gene expression rhythms (BMAL1, PER2, NR1D1) in the liver and suprachiasmatic nucleus (By similarity).
• Subcellular localization includes nucleus and cytoplasm; detected at protein level in neutrophils; contains zinc-finger domains and undergoes ubiquitination.

Experimental Guidance and Technical Tips

• For Western blot (WB), consider using whole cell lysates or nuclear extracts from relevant cell lines or tissues (e.g., neutrophils, liver) to detect endogenous EGR1. The predicted molecular weight is approximately 58 kDa, so use appropriate molecular weight markers and loading controls. Validate the antibody in the specific sample system of interest.
• For ELISA, consider optimizing the coating antigen concentration and antibody dilution using recombinant EGR1 protein or peptide. The immunogen corresponds to amino acids 1-250, so this region may be targeted.
• Cross-reactivity has been reported with human, mouse, and rat; however, individual validation in the relevant species and application is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw material sourcing, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target procurement. For EGR1-related research in oncology, inflammation, and circadian biology, CamelBio streamlines your supply chain from discovery to clinical applications.