Anti-EGR1 Rabbit Monoclonal Antibody for WB, IP, IF-P, IHC-P, ELISA - P18146

Core Product Specifications and Parameters

Biological Background: EGR1 Function and Localization

• EGR1 (Early growth response protein 1), also known as NGFI-A, Zif268, Krox-24, or AT225, is a zinc-finger transcription factor encoded by the EGR1 gene (GeneID: 1958) and belongs to the EGR family of immediate-early gene products.
• EGR1 functions as a transcriptional regulator by recognizing and binding the consensus EGR-site (5'-GCG(T/G)GGGCG-3') in target gene promoters; this binding occurs independently of cytosine methylation.
  Related references:
  PMID:25258363
  PMID:25999311
• It plays a dual role in cellular outcomes, promoting survival and proliferation while also activating tumor suppressors like p53/TP53 and TGFB1 to help avert malignant transformation.
• EGR1 is required for normal mitotic progression and hepatocyte regeneration following partial hepatectomy; it also mediates inflammatory processes and tissue damage after ischemic events by regulating cytokines such as IL1B and CXCL2.
• The protein localizes to both the nucleus and cytoplasm, where it undergoes various post-translational modifications including phosphorylation, ubiquitination, and SUMOylation (Ubl conjugation) that modulate its stability and transcriptional activity.
• EGR1 is detected in neutrophils at the protein level and contributes to circadian clock regulation by controlling the expression amplitude of core clock genes (e.g., BMAL1, PER2) in the liver and suprachiasmatic nucleus.

Experimental Guidance and Technical Tips

• The immunogen (aa 90–250 of human EGR1) covers key functional domains, including part of the activation domain and the zinc-finger DNA-binding region, suggesting high specificity for native EGR1 in multiple applications.
• For Western blot, a major band near 58 kDa is anticipated; additional bands may reflect post-translationally modified EGR1 or detection of related family members, and users should verify specificity using appropriate controls.
• In immunostaining (IHC-P, IF-P), anticipate both nuclear and cytoplasmic signals consistent with EGR1’s bimodal subcellular distribution; staining patterns may vary with cell type and activation status.
• Although cross-reactivity with mouse and rat EGR1 is reported, optimal working conditions (dilution, blocking, antigen retrieval) should be empirically determined for each sample matrix and application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to IVD raw materials, including optimized monoclonal antibodies such as this EGR1 rabbit monoclonal antibody, which supports investigations into transcription regulation, oncology, and circadian biology. As a comprehensive sourcing partner, we offer validated antibody pairs, bulk ancillary reagents, and customized solutions for rare-target raw-material needs, helping you accelerate assay development from concept to clinic.