Anti-EGLN1/EGLN2 Rabbit Polyclonal Antibody for WB, ELISA - Q9GZT9/Q96KS0

Core Product Specifications and Parameters

Biological Background: EGLN1/EGLN2 Function and Localization

• EGLN1 (PHD2) and EGLN2 (PHD1) are prolyl hydroxylase domain-containing proteins that function as cellular oxygen sensors, catalyzing post-translational hydroxylation of proline residues in hypoxia-inducible factor (HIF) alpha subunits under normoxic conditions.
• Hydroxylation targets HIF1A and HIF2A at specific oxygen-dependent degradation domains, leading to recognition by the von Hippel-Lindau (VHL) E3 ubiquitin ligase complex and subsequent proteasomal degradation.
• Under hypoxia, hydroxylation activity is attenuated, allowing HIFs to escape degradation, translocate to the nucleus, and induce transcription of genes involved in angiogenesis, erythropoiesis, and metabolic adaptation.
• EGLN1 is the predominant isoform under normoxia and plays critical roles in retinal and cardiac angiogenesis, heart function, and systemic oxygen homeostasis.
• The protein localizes to both the cytoplasm and nucleus, consistent with its role in sensing oxygen and regulating HIF stability.
• EGLN1 contains a zinc-finger domain and requires iron (Fe2+) and vitamin C as cofactors for its dioxygenase activity; it is regulated by acetylation, phosphorylation, and S-nitrosylation.
• This antibody recognizes both EGLN1 and EGLN2, which share significant sequence homology within the immunogen region (amino acids 250-350).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide derived from a conserved region; consider peptide blocking or competition assays to verify target specificity in your experimental system.
• For Western blotting, multiple bands may be observed corresponding to different isoforms or post-translational modifications; validate band identities using appropriate controls.
• For ELISA, the antibody may serve as capture or detection reagent, but optimal dilution and pairing with other reagents must be determined empirically.
• Since the antibody is polyclonal, lot-to-lot consistency may vary; qualify each new lot in your assay before routine use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop platform for IVD raw materials, offering a comprehensive portfolio of validated antibodies, antigen pairs, and ancillary reagents. This anti-EGLN1/EGLN2 antibody supports hypoxia research and assay development, and our team can assist with bulk sourcing, technical consultation, and custom procurement to accelerate your diagnostic projects from concept to clinic.