Anti-EGFR Rabbit Monoclonal Antibody for WB, ELISA - P00533

Core Product Specifications and Parameters

Biological Background: EGFR Function and Localization

• Receptor tyrosine kinase that binds EGF family ligands (EGF, TGFA, AREG, epigen/EPGN, BTC, epiregulin/EREG, HBEGF) triggering receptor homo/heterodimerization and autophosphorylation (PubMed:10805725, PubMed:27153536, PubMed:2790960, PubMed:12297049, PubMed:15611079, PubMed:17909029). Related references: PMID:10805725, PMID:27153536, PMID:2790960, PMID:12297049, PMID:15611079, PMID:17909029
• Activates downstream signaling cascades including RAS-RAF-MEK-ERK, PI3K-AKT, PLCgamma-PKC, and STATs modules (PubMed:27153536), and may activate NF-kappa-B (PubMed:11116146). Related references: PMID:27153536, PMID:11116146
• Directly phosphorylates other proteins such as RGS16 (PubMed:11602604) and MUC1 (PubMed:11483589), and positively regulates cell migration via interaction with CCDC88A/GIV (PubMed:20462955). Related references: PMID:11602604, PMID:11483589, PMID:20462955
• Isoform 2 may act as an antagonist of EGF action.
• Subcellular localization includes cell membrane, endoplasmic reticulum membrane, Golgi apparatus membrane, nucleus membrane, endosome, and secreted.
• Ubiquitously expressed; isoform 2 is also expressed in ovarian cancers.
• Plays a role in enhancing learning and memory performance and in mammalian pain signaling (by similarity).
• Acts as a receptor for hepatitis C virus (HCV) entry, facilitating CD81-CLDN1 receptor complex formation.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 363-463) lies within the extracellular domain of human EGFR; this region is well-suited for detecting total EGFR protein.
• For Western blot, use reducing conditions and validate with appropriate positive and negative cell lysates.
• For ELISA, consider both direct coating and sandwich formats; confirm specificity with recombinant EGFR protein.
• The antibody is rabbit monoclonal, ensuring high batch-to-batch consistency; perform internal validation for each new assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio enables diagnostic manufacturers and research laboratories to source validated IVD raw materials efficiently. This anti-EGFR monoclonal antibody supports oncology biomarker detection and signaling pathway studies. Our comprehensive portfolio encompasses optimized antibody pairs, monoclonal/polyclonal reagents, and bulk ancillary materials, with dedicated support for custom and rare-target sourcing requests.