Anti-EGFR Rabbit Monoclonal Antibody for FC - P00533

Core Product Specifications and Parameters

Biological Background: EGFR Function and Localization

• Epidermal growth factor receptor (EGFR), also known as ErbB-1 or HER1, is a receptor tyrosine kinase belonging to the ErbB family. Multiple isoforms exist due to alternative splicing; isoform 2 may function as an antagonist of EGF signaling.
• EGFR binds a variety of EGF family ligands, including EGF, TGFA, AREG, EPGN, BTC, EREG, and HBEGF, triggering receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. Related references: PMID:10805725 PMID:27153536 PMID:2790960 PMID:12297049 PMID:15611079 PMID:17909029
• Ligand-activated EGFR initiates multiple downstream signaling cascades, including RAS-RAF-MEK-ERK, PI3K-AKT, PLCgamma-PKC, and STAT pathways, and may also activate NF-kappa-B signaling. Related references: PMID:27153536 PMID:11116146
• EGFR directly phosphorylates other proteins such as RGS16 and MUC1, linking its activity to G protein-coupled receptor signaling and cell adhesion through beta-catenin. Related references: PMID:11602604 PMID:11483589
• Positively regulates cell migration by interacting with CCDC88A/GIV, which retains EGFR at the cell membrane following stimulation. It also plays a role in learning, memory, and pain signaling. Related references: PMID:20462955
• Subcellular localization includes the cell membrane, endoplasmic reticulum, Golgi apparatus, nuclear membrane, endosomes, and nucleus; a secreted form also exists.
• EGFR is a glycoprotein with tyrosine kinase activity, subject to multiple post-translational modifications including phosphorylation, ubiquitination, palmitoylation, and methylation. It serves as a host cell receptor for hepatitis C virus entry.
• Ubiquitously expressed in human tissues, with isoform 2 found in ovarian cancers.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 335–525 of human EGFR, corresponding to the extracellular domain, making this antibody suitable for detecting cell surface EGFR by flow cytometry.
• For flow cytometry, titrate the antibody to determine optimal working concentration and include appropriate isotype controls.
• Validate specificity in your cellular system, considering EGFR’s multiple isoforms and potential shedding of the extracellular domain.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents for EGFR-related diagnostics and research. Whether you need rare target reagents or scaled-up production, our one-stop platform bridges your concept to clinical application.