Anti-EGFR (Phospho-Y1173) Rabbit Monoclonal Antibody for WB, ELISA - P00533

Core Product Specifications and Parameters

Biological Background: EGFR Function and Localization

• Receptor tyrosine kinase that binds EGF family ligands (including EGF, TGFA, AREG, HBEGF) and triggers homo-/heterodimerization and autophosphorylation, converting extracellular cues into cellular responses. Related references: PMID:10805725 PMID:27153536 PMID:2790960 PMID:12297049 PMID:15611079 PMID:17909029
• Activates major downstream signaling cascades including RAS-RAF-MEK-ERK, PI3K-AKT, PLCgamma-PKC, STATs, and NF-kappa-B, regulating cell proliferation, survival, and differentiation. Related references: PMID:27153536 PMID:11116146
• Directly phosphorylates substrates such as RGS16 and MUC1, linking EGF receptor signaling to G protein-coupled pathways and modulating cell-matrix interactions. Related references: PMID:11602604 PMID:11483589
• Isoform 2, which lacks the transmembrane domain, may act as an antagonist of EGF action; it is notably expressed in ovarian cancers.
• Positively regulates cell migration through interaction with CCDC88A/GIV, retaining EGFR at the cell membrane and sustaining signaling. Related references: PMID:20462955
• Localizes to multiple subcellular compartments including the cell membrane, endoplasmic reticulum, Golgi, endosomes, nucleus, and can be secreted; this dynamic trafficking influences signal output.
• Acts as a host cell receptor for hepatitis C virus (HCV) entry, facilitating CD81-CLDN1 complex formation and membrane fusion.
• Ubiquitously expressed with extensive post-translational modifications (e.g., phosphorylation, glycosylation, ubiquitination, palmitoylation) that fine-tune its activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a phospho-specific peptide surrounding tyrosine 1173 of human EGFR; antibody reactivity is phosphorylation-state dependent.
• For western blot, consider including both phospho-specific and total EGFR antibodies as controls to normalize phosphorylation levels and confirm signal specificity.
• Validate antibody performance in the relevant biological sample system; strong signals may require optimized lysis buffers containing phosphatase inhibitors.
• For ELISA, ensure the coating antigen or capture antibody does not mask the Y1173 phospho-epitope.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials, including highly specific monoclonal antibodies for key signaling targets such as EGFR. Our validated phospho-specific antibodies, ancillary reagents, and flexible sourcing options support oncology and signal transduction research, enabling streamlined diagnostic development. Explore our offerings to accelerate your projects from concept to clinic with reliable, one-stop procurement.