Anti-EGFR (Phospho Y1068) Rabbit Monoclonal Antibody for WB and ELISA - P00533

Core Product Specifications and Parameters

Biological Background: EGFR Function and Localization

• EGFR is a receptor tyrosine kinase that binds EGF family ligands, triggering receptor dimerization and autophosphorylation on key cytoplasmic residues, including Y1068, to activate downstream signaling (PubMed:10805725, PubMed:27153536, PubMed:2790960). Related references: PMID:10805725, PMID:27153536, PMID:2790960
• The phosphorylated receptor recruits adaptor proteins such as GRB2, leading to activation of at least four major signaling cascades: RAS-RAF-MEK-ERK, PI3K-AKT, PLCγ-PKC, and STAT modules (PubMed:27153536). Related references: PMID:27153536
• EGFR directly phosphorylates other proteins including RGS16 and MUC1, linking EGF signaling to GPCR signaling and cell adhesion pathways (PubMed:11602604, PubMed:11483589). Additionally, it can activate the NF-kappa-B signaling cascade (PubMed:11116146). Related references: PMID:11602604, PMID:11483589, PMID:11116146
• Interaction with CCDC88A/GIV retains EGFR at the cell membrane, promoting cell migration (PubMed:20462955). Related references: PMID:20462955
• Subcellularly, EGFR is localized to the cell membrane, endoplasmic reticulum, Golgi apparatus, nucleus, endosomes, and can be secreted. This dynamic distribution supports its multifaceted signaling roles.
• Ubiquitously expressed, with isoform 2 also detected in ovarian cancers. EGFR also functions as a host receptor for hepatitis C virus (HCV) entry in hepatocytes.
• Key post-translational modifications include phosphorylation (especially at Y1068 after ligand binding), glycosylation, ubiquitination, and palmitoylation, essential for its kinase activity and signaling duration.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide corresponding to the Y1068 region; for WB, ensure inclusion of phosphatase inhibitors in lysis buffers to preserve phosphorylation.
• For ELISA, coating with the phosphorylated peptide antigen may help assess antibody specificity; a non-phosphorylated peptide can serve as a negative control.
• This antibody has shown cross-reactivity with human and mouse EGFR, so it can be applied to studies in both species; however, validation in the target sample system is recommended.
• Consider using a loading control that is not affected by phosphorylation status to normalize WB signals.
• Given the 134 kDa molecular weight, adjust gel percentage and transfer conditions appropriately.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing bridge for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Our portfolio includes phospho-specific antibodies like this anti-EGFR (Phospho Y1068) rabbit monoclonal antibody, designed to support your EGFR signaling and oncology research. From concept to clinic, CamelBio provides reliable raw materials and technical consulting to accelerate your diagnostic assay development.