Anti-EGFR (L858R) Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P00533

Core Product Specifications and Parameters

Biological Background: EGFR Function and Localization

• EGFR is a proto-oncogene member of the ErbB family of receptor tyrosine kinases, also known as HER1 or ErbB-1.
• Binds a variety of EGF-like ligands (e.g., EGF, TGF-alpha, HB-EGF), leading to receptor dimerization, autophosphorylation, and activation of major signaling cascades including RAS-RAF-MEK-ERK, PI3K-AKT, PLCgamma-PKC, and STATs. Related references: PMID:10805725, PMID:27153536, PMID:2790960, PMID:12297049, PMID:15611079, PMID:17909029
• Activates the NF-kappa-B signaling cascade. Related references: PMID:11116146
• Directly phosphorylates RGS16 and MUC1, coupling EGFR signaling to G-protein signaling and beta-catenin pathways, respectively. Related references: PMID:11602604, PMID:11483589
• Regulates cell migration via interaction with CCDC88A/GIV, which retains EGFR at the cell membrane and promotes signaling. Related references: PMID:20462955
• Isoform 2 may act as an antagonist of EGF action.
• Predominantly localizes to the cell membrane, but is also found in endoplasmic reticulum, Golgi apparatus, nucleus, endosomes, and secreted form.
• Ubiquitously expressed; isoform 2 is also detected in ovarian cancers.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a peptide within amino acids 800–900 of EGFR containing the L858R mutation; confirm specificity for the mutant by comparing reactivity against wild-type EGFR.
• For Western blot, use denaturing and reducing conditions; expect a band near 134 kDa. Validate using cell lysates expressing EGFR L858R and wild-type controls.
• For IHC-P, optimize antigen retrieval and include tissue known to harbor the L858R mutation (e.g., lung adenocarcinoma) as a positive control.
• For ELISA, consider using the immunizing peptide or recombinant EGFR L858R protein as a standard, and assess potential cross-reactivity with wild-type EGFR.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with a streamlined sourcing solution for high-quality IVD raw materials in oncology. For EGFR mutation-specific antibodies, we supply validated monoclonal antibodies, bulk ancillary reagents, and expert technical support to accelerate your assay development from concept to clinic. Our one-stop service ensures reliable access to critical raw materials for precision oncology research.