Anti-EGFL7 Rabbit Polyclonal Antibody for WB - Q9UHF1

Core Product Specifications and Parameters

Biological Background: EGFL7 Function and Localization

• Epidermal growth factor-like protein 7 (EGFL7), also referred to as Multiple EGF-like domains protein 7, NOTCH4-like protein, Zneu1, or Vascular endothelial statin (VE-statin), is a secreted glycoprotein characterized by the presence of multiple EGF-like domains and a coiled-coil region.
• The protein contains calcium-binding EGF-like domains and disulfide bonds, which are critical for its structural integrity and interactions with the extracellular matrix.
• EGFL7 plays a pivotal role in vascular development by regulating tubulogenesis, a key process in blood vessel formation, and promotes endothelial cell adhesion and angiogenesis.
• It inhibits smooth muscle cell migration induced by platelet-derived growth factor (PDGF)-BB, suggesting a role in maintaining vascular wall stability.
• Localized to the extracellular space, EGFL7 is secreted and functions as a paracrine factor in the vascular microenvironment.
• As indicated by its functional keywords, EGFL7 is involved in cell adhesion, developmental processes, and differentiation, underscoring its importance in embryonic vasculogenesis and tissue remodeling.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 24–100 of human EGFL7, a region likely encompassing the N-terminal EGF-like domain; users should consider that epitope recognition may be influenced by the protein’s folded structure or glycosylation.
• For Western blot, the predicted molecular weight of EGFL7 is approximately 30 kDa; however, post-translational modifications may cause slight shifts, so it is advisable to validate the band size in relevant endothelial cell lysates.
• Cross-reactivity with mouse and rat suggests that the epitope is conserved across these species, making the antibody suitable for comparative studies in disease models.
• In ELISA applications, consider optimizing coating conditions and antibody concentrations using recombinant EGFL7 protein or conditioned media from vascular cell lines.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive procurement partner for diagnostic manufacturers, laboratories, and research institutes, offering a reliable supply of validated IVD raw materials. This anti-EGFL7 polyclonal antibody supports angiogenesis and vascular biology research applications. We facilitate one-stop access to bulk ancillary reagents, optimized antibody pairs, and tailored sourcing for rare-target raw materials, ensuring seamless integration from discovery to clinical development without the need for multiple vendor logistics.