Anti-EGF Rabbit Polyclonal Antibody for WB, DB, ELISA - P01133

Core Product Specifications and Parameters

Biological Background: EGF Function and Localization

• EGF, or Pro-epidermal growth factor, is a membrane-bound growth factor that can be processed into soluble forms to signal through the EGF receptor (EGFR).
• EGF stimulates growth of epidermal and epithelial tissues in vivo and in vitro, and also acts on some fibroblasts.
• It functions as a magnesiotropic hormone, enhancing renal magnesium reabsorption by activating TRPM6 channels via EGFR in the distal convoluted tubule.
• EGF can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro. Related references: PMID:10964941
• Subcellularly, EGF is localized to the membrane as a transmembrane precursor.
• Tissue expression includes kidney, salivary gland, cerebrum, and prostate.
• The protein contains EGF-like domains, multiple disulfide bonds, and is glycosylated, indicating structural complexity.
• The gene is subject to alternative splicing, and mutations are linked to primary hypomagnesemia.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 971-1023, a region within the C-terminal part of the EGF precursor. This sequence is conserved between human and mouse (as indicated by cross-reactivity), making the antibody suitable for both species in applications like WB, DB, and ELISA.
• For Western blotting, the full-length pro-EGF protein runs at approximately 134 kDa, but processed fragments may also be detected. Consider using appropriate positive controls (e.g., kidney or salivary gland lysates) and optimizing blocking conditions.
• In ELISA, the antibody may be used for antigen capture or detection; validate pairing with your secondary system and check for specificity against recombinant EGF.
• As a polyclonal antibody, lot-to-lot variability should be assessed; performing titration experiments is recommended to determine optimal working dilutions for your specific experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies diagnostic manufacturers and research laboratories with high-quality IVD raw materials, technical services, and consulting. This anti-EGF polyclonal antibody supports investigations into growth factor signaling, renal electrolyte transport, and cancer biology. Beyond this product, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to accelerate your development from concept to clinic.