Anti-EGF Polyclonal Antibody for WB, IF-P, ELISA - P01133

Core Product Specifications and Parameters

Biological Background: EGF Function and Localization

• EGF is synthesized as a membrane-anchored precursor protein (Pro-epidermal growth factor) that is proteolytically processed to release mature EGF, which contains multiple EGF-like domains and functions as a potent growth factor.
• EGF stimulates the proliferation and differentiation of epidermal and epithelial tissues both in vivo and in vitro, and also promotes the growth of certain fibroblasts in cell culture.
• Acts as a magnesiotropic hormone in the kidney, enhancing magnesium reabsorption in the distal convoluted tubule through EGFR-mediated activation of the TRPM6 magnesium channel.
• Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro, demonstrating cross-species neurotrophic activity. Related references: PMID:10964941
• Localized primarily to the cell membrane as a transmembrane protein, with its ectodomain shed to generate soluble mature EGF.
• Expressed in kidney, salivary gland, cerebrum, and prostate, with additional tissue-specific roles suggested by its presence in these organs.
• Contains characteristic EGF-like domain repeats, disulfide bonds, and glycosylation sites; classified under growth factors and transmembrane proteins in key biological databases.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 851–950 of human EGF (accession NP_001954.2), residing in the C-terminal region of the proprotein. This region may be retained in the membrane-bound precursor and some processed forms.
• For Western blotting (WB), expect major bands corresponding to the full-length precursor (approximately 134 kDa) and possibly lower molecular weight species representing cleavage products or mature EGF. Validate in EGF-expressing samples such as kidney, salivary gland, or prostate tissues.
• In immunofluorescence (IF-P), staining should localize to the plasma membrane, consistent with the membrane-bound precursor. Permeabilization and fixation conditions should be optimized for the specific sample system.
• For ELISA applications, consider pairing this polyclonal antibody with validated capture or detection reagents. Test serial dilutions to establish optimal working concentrations for your specific assay format.
• Cross-reactivity with mouse and rat EGF has been reported; however, antibody performance in these species should be verified individually under your experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to high-quality IVD raw materials, including validated antibody pairs, bulk polyclonal and monoclonal antibodies, and ancillary reagents. The Anti-EGF Polyclonal Antibody (P01133) targets a critical growth factor involved in cell proliferation and tissue regeneration, making it valuable for multiple diagnostic and research applications. Whether you need scale-up sourcing of rare-target antibodies or comprehensive technical support, CamelBio streamlines procurement from concept to clinical application.