Anti-EGF Monoclonal Antibody for WB - P01132

Core Product Specifications and Parameters

Biological Background: EGF Function and Localization

• EGF is a growth factor that stimulates the proliferation and differentiation of epidermal and epithelial tissues in vivo and in vitro, as well as some fibroblasts in cell culture.
• It functions as a magnesiotropic hormone, promoting magnesium reabsorption in the renal distal convoluted tubule by activating the magnesium channel TRPM6 through EGFR engagement (By similarity).
• The protein is synthesized as a membrane-bound precursor (Pro-epidermal growth factor) that undergoes proteolytic cleavage to release the mature soluble growth factor.
• Subcellular localization includes the cell membrane, where the precursor is anchored before processing.
• Contains EGF-like domains and multiple disulfide bonds that are critical for its tertiary structure and receptor binding.
• Classified under key biological processes such as growth factor activity, transmembrane signaling, and magnesium homeostasis.

Experimental Guidance and Technical Tips

• For WB, use mouse tissue lysates known to express EGF, such as kidney or salivary gland. Expect a band at ~133 kDa for Pro-EGF; additional lower bands may represent processed forms.
• For ELISA, this antibody can be utilized in direct or indirect ELISA formats; pairing with a secondary antibody suitable for rabbit IgG or a matched detection antibody is recommended.
• Since the immunogen corresponds to the C-terminal EGF domain, the antibody likely recognizes both the precursor and mature EGF. Validate in the relevant sample system.
• Optimize blocking and incubation conditions to reduce non-specific binding, especially when using mouse tissues.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive sourcing partner for IVD raw materials, providing high-quality antibodies like this EGF monoclonal antibody for research in cell growth and magnesium homeostasis. We support diagnostic manufacturers with validated antibody pairs, bulk reagent supply, and custom sourcing of rare targets, streamlining your development from concept to clinic.