Anti-EG5/KIF11 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P52732

Core Product Specifications and Parameters

Biological Background: EG5/KIF11 Function and Localization

• KIF11 (Eg5) is a kinesin-like motor protein essential for mitosis: it drives bipolar spindle formation and chromosome congression. Related references: PMID:19001501, PMID:37728657
• In interphase, it mediates Golgi-derived secretory vesicle trafficking to the cell surface. Related references: PMID:23857769
• Subcellular localization is predominantly cytoplasmic, with enrichment at spindle poles during mitosis.
• The protein contains ATP-binding, coiled-coil, and motor domains, characteristic of kinesin heavy chains.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination, as indicated by UniProt keywords.
• Encoded by the human KIF11 gene (Gene ID: 3832), the protein has a predicted molecular weight of 119 kDa (UniProt accession P52732).
• Mutations in KIF11 are linked to disease variants, underscoring its critical role in cell division.

Experimental Guidance and Technical Tips

• The immunogen comprises residues 797–1056 of human EG5/KIF11; this segment may yield antibodies with reduced cross-reactivity to other kinesin-like proteins. Consider verifying specificity via sequence alignment.
• For Western blotting, expect a band at approximately 119 kDa. Validate band identity using appropriate controls, such as KIF11-overexpressing or knockdown cell lysates.
• In immunofluorescence/immunocytochemistry, check for spindle pole localization in mitotic cells. Include a mitotic enrichment step (e.g., serum starvation or nocodazole treatment) to enhance detection.
• For ELISA applications, perform coating and pairing optimization under your assay conditions. The antibody may be useful for antigen capture or detection in sandwich immunoassays.
• Cross-reactivity data indicate specificity for human EG5; test other species as needed if expanding the application scope.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, from concept to clinic. For EG5/KIF11-related research and diagnostic development, we offer validated polyclonal antibodies, monoclonal alternatives, and ancillary reagents to support your workflows. Leverage our supply chain expertise to secure high-quality, bulk raw materials for rare and critical targets like kinesin motor proteins.