Anti-EG5/KIF11 Rabbit Monoclonal Antibody for WB & ELISA - P52732

Core Product Specifications and Parameters

Biological Background: EG5/KIF11 Function and Localization

• KIF11, also known as Eg5, HKSP, KNSL1, and TRIP5, is a kinesin-like motor protein that belongs to the kinesin-5 family.

• It plays a critical role in mitosis by establishing a bipolar spindle and facilitating chromosome congression during cell division.

  Related references: PMID:19001501 PMID:37728657

• In interphase, KIF11 contributes to the transport of secretory proteins from the Golgi complex to the cell surface.

  Related references: PMID:23857769

• The protein localizes predominantly to the cytoplasm and spindle poles, associating with the cytoskeleton and mitotic spindle apparatus.

• KIF11 is subject to multiple post-translational modifications, including acetylation, phosphorylation, and ubiquitination (isopeptide bond and Ubl conjugation), which regulate its motor activity and stability.

• Mutations in KIF11 are linked to microcephaly with or without lymphedema and chorioretinopathy (MCLMR), highlighting its importance in human development.

Experimental Guidance and Technical Tips

• For Western blotting, consider using a positive control such as HeLa or other dividing human cells where KIF11 is highly expressed.
• The immunogen region (aa 900–1056) lies within the C-terminal domain; to avoid potential cross-reactivity with other kinesins, validate specificity in appropriate knockout or knockdown models.
• In ELISA, optimal coating buffers and antibody titers should be determined empirically for each application.
• Given the large molecular weight (119 kDa), ensure sufficient gel separation and transfer conditions for immunoblotting.
• This product is recommended for research use; performance in diagnostic assays must be validated under intended-use conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive range of IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and ancillary reagents for diagnostic development. For researchers focused on cell cycle and mitosis targets such as KIF11/Eg5, we offer bulk sourcing and custom solutions to accelerate your assay workflow. Contact us to explore our full catalog and technical support services.