Anti-EFTUD2 Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - Q15029

Core Product Specifications and Parameters

Biological Background: EFTUD2 Function and Localization

• EFTUD2 (116 kDa U5 snRNP component, SNU114, also known as elongation factor Tu GTP-binding domain-containing protein 2) is a GTP-binding protein that serves as a structural and functional subunit of the U5 small nuclear ribonucleoprotein (snRNP), essential for pre-mRNA splicing. Related references: PMID:16723661
• It is integral to the U4/U6-U5 tri-snRNP complex, a building block of the major spliceosome, and is required for pre-catalytic, catalytic, and post-catalytic spliceosome assembly and disassembly. Related references: PMID:25092792, PMID:28076346, PMID:28502770
• EFTUD2 also functions in the minor spliceosome and is probably involved in the splicing of U12-type introns in pre-mRNAs.
• The protein localizes exclusively to the nucleus, consistent with its primary role in mRNA processing.
• Post-translational modifications include acetylation and phosphorylation, and it is subject to alternative splicing and ubiquitin conjugation.
• Mutations in EFTUD2 are linked to mandibulofacial dysostosis with microcephaly (MFDGA), highlighting its developmental importance.

Experimental Guidance and Technical Tips

• For Western blot analysis, the expected molecular weight of EFTUD2 is approximately 109 kDa (theoretical) or 116 kDa (observed), so ensure proper gel percentage and transfer conditions.
• In immunofluorescence and immunohistochemistry, anticipate nuclear staining; include DAPI co-staining to confirm localization.
• For immunoprecipitation, this polyclonal antibody can efficiently pull down native EFTUD2 from human, mouse, and rat cell lysates; consider cross-linking antibody to beads for cleaner results.
• Because this is a polyclonal antibody, we recommend validating each new lot in your specific assay system and including appropriate positive and negative controls.
• The immunogen (N-terminal 1–205 amino acids) may not recognize isoforms lacking this region; verify target expression in your model.
• Optimize antibody dilutions and blocking conditions for each application, as performance can vary between techniques and sample types.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated sourcing partner for IVD raw materials, providing validated antibodies like anti-EFTUD2 to support spliceosome research and diagnostic assay development. We supply bulk ancillary reagents, optimized antibody pairs, and custom monoclonal/polyclonal antibody sourcing, helping you move seamlessly from concept to clinic. Reach out to our team to discuss your specific procurement needs.