Anti-EFS Rabbit Polyclonal Antibody for WB and ELISA - O43281

Core Product Specifications and Parameters

Biological Background: EFS Function and Localization

• EFS (Embryonal Fyn-associated substrate), also known as Cas scaffolding protein family member 3 (CASS3), is a docking protein encoded by the EFS gene.
• It plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion, potentially serving as both an activator of SRC and a downstream effector (By similarity).
• The protein interacts with the SH3 domain of FYN and with CRK, SRC, and YES, facilitating signal transduction cascades.
• EFS belongs to the CAS (Crk-associated substrate) scaffolding protein family, characterized by SH3 domain binding and multi-site phosphorylation.
• It undergoes alternative splicing, producing multiple isoforms that may have distinct functional roles.
• The protein has been detected in lung and placenta tissues, suggesting tissue-specific signaling roles in these organs.
• Keywords associated with EFS include cell adhesion, phosphoprotein, SH3 domain, and SH3-binding, emphasizing its role in adhesion-related phosphotyrosine signaling.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 60-190 of human EFS, a region likely to be conserved; it may be suitable for detecting EFS in human and mouse samples (cross-reactivity confirmed with mouse).
• For Western blot (WB), consider using a loading control from the expected molecular weight of approximately 59 kDa, and validate the antibody in a relevant cell line or tissue lysate.
• For ELISA, consider pairing this polyclonal antibody with a matched monoclonal antibody to improve assay specificity, and include appropriate controls to account for potential batch-to-batch variability inherent to polyclonals.
• Due to the "by similarity" functional annotations, confirm the activation and interaction partners in your specific experimental system through co-immunoprecipitation or similar assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop sourcing partner for IVD raw materials, supplying validated monoclonal and polyclonal antibodies, optimized antibody pairs, and bulk ancillary reagents to diagnostic manufacturers and research laboratories worldwide. This anti-EFS polyclonal antibody supports cell adhesion and tyrosine kinase signaling research, and CamelBio can also assist with sourcing rare-target antibodies and developing customized immunoassay solutions. From concept to clinic, CamelBio streamlines your raw-material procurement with technical consultation and flexible supply chains.