Anti-EFNB1 Rabbit Polyclonal Antibody for WB, ELISA - P98172

Core Product Specifications and Parameters

Biological Background: EFNB1 Function and Localization

• EFNB1 encodes Ephrin-B1, a transmembrane ligand for Eph receptor tyrosine kinases, and is a member of the ephrin family.
• It binds to Eph receptors (EPHB1, EPHB2, EPHB3) to trigger contact-dependent bidirectional signaling, playing critical roles in cell migration, repulsion, and adhesion during neuronal, vascular, and epithelial development.
  Related references: PMID:7973638 and PMID:8070404
• In neuronal development, Ephrin-B1 induces commissural axon growth cone collapse and may constrain longitudinal axon orientation (By similarity).
• Subcellular localization includes the cell membrane, membrane rafts, and the nucleus, consistent with its role in signal transduction.
• EFNB1 is widely expressed, with particularly high levels in retina, sciatic nerve, heart, and spinal cord.
  Related references: PMID:7973638
• Post-translational modifications such as phosphorylation and glycosylation, along with disulfide bonds and a transmembrane domain, are essential for its function.
• Mutations in EFNB1 are linked to craniosynostosis (craniofrontonasal syndrome), highlighting its importance in skeletal development.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the intracellular domain (amino acids 259–346); consider how epitope accessibility may influence detection in various assay formats.
• For Western blot, the predicted molecular weight is approximately 38 kDa; reduce and denature samples and validate the band size in your system.
• Cross-reactivity with mouse has been reported; if using mouse tissues, validate the antibody under your specific experimental conditions.
• As a polyclonal antibody, include appropriate controls (e.g., blocking peptide, knockout lysate) to confirm specificity.
• For ELISA, test both direct and indirect formats and optimize coating conditions to achieve the best signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, from discovery to clinic. This anti-EFNB1 antibody supports neuroscience and developmental biology research, and we can also supply validated antibody pairs, bulk ancillary reagents, and rare-target raw materials to meet your diagnostic development needs. Contact us to streamline your reagent procurement and accelerate your projects.