Anti-EFNA1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P20827

Core Product Specifications and Parameters

Biological Background: EFNA1 Function and Localization

• EFNA1 encodes ephrin-A1, a glycosylphosphatidylinositol (GPI)-anchored cell surface ligand of the Eph receptor tyrosine kinase family. It is also known as LERK-1, B61, and TNF alpha-induced protein 4.
• Ephrin-A1 binds promiscuously to Eph receptors on adjacent cells, mediating contact-dependent bidirectional signaling that regulates cell migration, repulsion, and adhesion during neuronal, vascular, and epithelial development. Related references: PMID:17332925 PMID:18794797
• In vascular biology, ephrin-A1 plays a critical role in angiogenesis by activating EPHA2 to promote endothelial cell differentiation and migration.
• As a ligand for EPHA3, ephrin-A1 inhibits epithelial-to-mesenchymal transition in cardiac cells, contributing to proper heart development. Related references: PMID:12794130
• Ephrin-A1 may also participate in dendritic spine morphogenesis (by similarity).
• The protein localizes predominantly to the cell membrane via a GPI anchor but can be released as a soluble form through cleavage. It is abundantly expressed in brain, and its expression is often down-regulated in primary glioma. Soluble monomeric ephrin-A1 has been detected in glioblastoma and breast cancer cells.
• Post-translational modifications include disulfide bond formation and N-linked glycosylation. The protein undergoes alternative splicing, generating membrane-bound and secreted isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 19-182 of human EFNA1, covering the extracellular receptor-binding domain. The resulting polyclonal antibody may recognize both full-length GPI-anchored and soluble isoforms.
• For Western blot, users may test antibody dilutions in the range commonly used for polyclonal antibodies (e.g., 1:500–1:2000) and confirm specificity using lysates from EFNA1-overexpressing and knockdown cell lines. Endogenous EFNA1 migrates at approximately 24 kDa.
• For IHC-P, consider optimizing antigen retrieval and include appropriate tissue controls. Both paraffin-embedded human and mouse tissue sections can be used, given the claimed cross-reactivity.
• In ELISA, the antibody can be employed as a capture or detection reagent; the optimal working concentration should be determined experimentally in the specific assay format.
• Due to the GPI-anchored nature and potential shedding of ephrin-A1, detectability may vary between cell types and treatment conditions; validation in the relevant sample system is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a one-stop sourcing solution for IVD raw materials, connecting diagnostic developers with validated antibody pairs, high-quality monoclonal and polyclonal antibodies, bulk ancillary reagents, and expert technical consultation. For EFNA1-related research in angiogenesis, cardiovascular development, and cancer, our portfolio includes anti-EFNA1 antibodies, recombinant proteins, and supporting reagents to streamline assay development from early-stage research to clinical application.