Anti-EFHD2 Polyclonal Antibody for WB, ELISA - Q96C19

Core Product Specifications and Parameters

Biological Background: EFHD2 Function and Localization

• EFHD2, also known as Swiprosin-1, is an EF-hand domain-containing protein encoded by the EFHD2 gene (Gene ID: 79180) in humans.
• It functions as a negative regulator of the canonical NF-kappa-B signaling pathway in B cells, modulating B-cell receptor (BCR)-induced apoptosis.
• EFHD2 controls spontaneous apoptosis through the regulation of BCL2L1 (also known as BCL-XL) protein abundance.
• The protein is localized to membrane rafts, suggesting a role in membrane-associated signaling platforms.
• EFHD2 is preferentially expressed in CD8+ T lymphocytes and is found in lymphocytes, indicating a role in immune cell function.
• As an EF-hand protein, EFHD2 binds calcium ions, which likely influence its conformation and activity; it is also subject to post-translational modifications including acetylation and phosphorylation.
• The molecular weight of the unmodified protein is approximately 27 kDa (26.7 kDa calculated from sequence), consistent with the band detected on Western blots.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal 78 amino acids of human EFHD2, which may include an EF-hand motif; consider this region's accessibility when designing detection strategies.
• For Western blot, the predicted molecular weight is ~27 kDa; use appropriate positive controls (e.g., human lymphocyte lysate or mouse tissues expressing Efhd2) and validate band specificity.
• In ELISA, the recombinant immunogen or native protein in cell lysates can serve as a capture or detection antigen; optimize coating conditions for best performance.
• Cross-reactivity with mouse is reported, so this antibody may be suitable for mouse models, but always verify reactivity and specificity in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is dedicated to supporting IVD developers with a comprehensive portfolio of high-quality antibodies and reagents. For researchers studying B-cell signaling, apoptosis, and immune regulation, we offer this EFHD2 polyclonal antibody along with validated antibody pairs, recombinant proteins, and ancillary reagents. Our one-stop sourcing model accelerates assay development from target discovery to diagnostic application.