Anti-EFHC1 Rabbit Polyclonal Antibody for WB, ELISA - Q5JVL4

Core Product Specifications and Parameters

Biological Background: EFHC1 Function and Localization

• EFHC1 is an EF-hand domain-containing protein (myoclonin-1) that serves as a microtubule inner protein (MIP) in ciliary and flagellar axonemes, critical for motile cilia beating. Related references: PMID:36191189

• It functions as a microtubule-associated protein regulating cell division and neuronal migration during cortical development. Related references: PMID:19734894 PMID:28370826

• EFHC1 is necessary for radial and tangential cell migration in the developing brain, modulating cell morphology and process formation. Related references: PMID:22926142

• The protein can enhance calcium influx via CACNA1E channels and stimulate programmed cell death. Related references: PMID:15258581 PMID:19734894 PMID:22926142

• Subcellularly, EFHC1 localizes to the cytoplasm, ciliary/flagellar axonemes, centrosomes, and mitotic spindle poles.

• It is widely expressed in tissues but is not detected in lymphocytes.

• Mutations in EFHC1 are linked to epilepsy, and the protein is subject to alternative splicing.

Experimental Guidance and Technical Tips

• For Western blot, expect a specific band around 74 kDa corresponding to the predicted molecular weight of EFHC1; validate with an appropriate loading control and positive control lysate (e.g., HeLa, SH-SY5Y cells).
• In ELISA, consider using the recombinant immunogen (amino acids 391–640) as a coating antigen or standard for quantitative detection.
• Given the reported cross-reactivity with human, mouse, and rat, this antibody is suitable for comparative studies across these species; verify signal specificity in each system.
• As a polyclonal antibody, it may recognize multiple epitopes and is robust for denaturing applications like WB; for native protein detection, optimize conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a premier one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers, laboratories, and research institutes from concept to clinic. For EFHC1-related research—including cilia biology, neuronal migration, and epilepsy—we supply validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and custom rare-target procurement. Our integrated services ensure seamless access to critical raw materials for assay development and production.