Anti-EFCAB3 Rabbit Polyclonal Antibody for WB, ELISA - Q8N7B9

Core Product Specifications and Parameters

Biological Background: EFCAB3 Function and Localization

• EFCAB3 (EF-hand calcium-binding domain-containing protein 3) is a protein characterized by the presence of EF-hand domains, which are helix-loop-helix structural motifs known to bind calcium ions.
• The gene EFCAB3 encodes this protein in humans, with multiple isoforms generated through alternative splicing, as indicated by UniProt annotation.
• The protein is classified as a phosphoprotein, suggesting regulation by phosphorylation, and contains repetitive sequence elements.
• It has been identified in large-scale proteomics studies, confirming its expression at the protein level.
• The predicted molecular weight of the canonical isoform is approximately 50 kDa, consistent with the observed 50 kDa band in western blot.
• No specific subcellular localization or tissue-specific expression data are currently available; however, EF-hand proteins often participate in calcium-dependent cellular signaling pathways.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region (amino acids 249–438) of human EFCAB3. This design may enable recognition of multiple isoforms if the sequence is conserved among splice variants.
• Verified applications include western blot (WB) and ELISA. For WB, a band near 50 kDa is expected; consider using appropriate positive and negative controls to confirm specificity.
• Cross-reactivity with mouse and rat samples has been reported, suggesting that the epitope is conserved across these species. Validate reactivity in your specific experimental system.
• Optimal working dilutions should be determined empirically. Refer to standard protocols for rabbit polyclonal antibodies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, laboratories, and research institutes with a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This EFCAB3 antibody supports studies in calcium-binding proteins and EF-hand domain research. For rare target sourcing or custom manufacturing requests, CamelBio's supply network and technical consulting services offer streamlined access to the materials needed from concept to clinic.