Anti-eEF2 Rabbit Polyclonal Antibody for WB and ELISA - P13639

Core Product Specifications and Parameters

Biological Background: eEF2 Function and Localization

• eEF2 (Elongation factor 2) is a key GTPase that drives ribosomal translocation during protein synthesis elongation. Related references: PMID:26593721
• It catalyzes the coordinated movement of tRNA molecules and mRNA through the ribosome, transitioning from pre-translocational to post-translocational states.
• eEF2 is encoded by the EEF2 gene, and mutations are linked to spinocerebellar ataxia 26 (SCA26), a neurodegenerative disorder.
• The protein is predominantly cytoplasmic but can also localize to the nucleus, suggesting possible extra-ribosomal functions.
• Post-translational modifications include phosphorylation, acetylation, ADP-ribosylation, methylation, and ubiquitination, which regulate its activity.
• It is a member of the GTP-binding elongation factor family and an essential component of the protein biosynthesis machinery.
• Its molecular weight is approximately 95 kDa (predicted 95,338 Da from sequence).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–290) of human eEF2; consider this region for epitope mapping or in competitive assays.
• For Western blot, use whole cell or tissue lysates; eEF2 is abundant, so standard loading (e.g., 20–30 µg) may suffice. Optimize blocking and antibody dilution for your system.
• In ELISA, coat with recombinant eEF2 or peptide and perform checkerboard titration to determine optimal coating and antibody concentrations.
• Validate cross-reactivity with human samples; reactivity with other species has not been tested, so empirically verify if using non-human samples.
• Because eEF2 is subject to multiple post-translational modifications, band shifts or multiple bands may appear under certain conditions; dephosphorylation or specific inhibitors may be used to identify modified forms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies like this anti-eEF2 antibody, bulk ancillary reagents, and rare target raw-material sourcing. Whether you are developing assays for protein synthesis studies or neurodegeneration biomarkers, our comprehensive portfolio and technical consulting can support your project from concept to clinic.