Anti-eEF1G Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P26641

Core Product Specifications and Parameters

Biological Background: eEF1G Function and Localization

• eEF1G, also known as eEF-1B gamma, EF1G, or GIG35, is the gamma subunit of the elongation factor-1 (eEF1) complex.
• It is involved in protein biosynthesis as an elongation factor (UniProt keyword: Elongation factor, Protein biosynthesis).
• UniProt annotates its molecular function as probably playing a role in anchoring the complex to other cellular components.
• Post-translational modifications include acetylation (keyword: Acetylation) and ubiquitination (keyword: Ubl conjugation).
• Alternative splicing generates multiple transcript isoforms (keyword: Alternative splicing).
• Tissue specificity: highly expressed in pancreatic tumor tissue; moderate expression in normal kidney, intestine, pancreas, stomach, lung, brain, spleen, and liver.
• The protein has been identified in several proteomics studies (keyword: Proteomics identification) and is part of the reference proteome (keyword: Reference proteome).
• The molecular weight of the canonical isoform is approximately 50 kDa (UniProt: 50119 Da; consistent with the observed 50 kDa band in SDS-PAGE).

Experimental Guidance and Technical Tips

• The immunogen (recombinant protein covering amino acids 168–437 of human eEF1G) spans a central region of the protein, likely generating antibodies that recognize the full-length native protein.
• For western blot (WB), expect a band around 50 kDa; use a positive control lysate (e.g., from pancreas or HeLa cells) to verify specificity.
• In immunohistochemistry (IHC-P) on formalin-fixed paraffin-embedded sections, pancreatic tumor tissue may serve as a suitable positive control based on expression data.
• For immunofluorescence (IF/ICC), optimize fixation and permeabilization conditions to preserve epitope recognition.
• As a polyclonal antibody, it may detect multiple isoforms or post-translationally modified variants; verify specificity using blocking peptides or knockout controls if available.
• Cross-reactivity with mouse and rat enables potential use in rodent models; validate performance in the specific tissue or cell type of interest.
• For ELISA applications, titrate the antibody against the recombinant immunogen or native protein to determine optimal working concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, including validated antibody pairs, optimized recombinant antibodies, and ancillary reagents for diagnostic assay development. For researchers targeting eEF1G, a component of the translation elongation machinery, we can supply this polyclonal antibody along with complementary products such as blocking peptides, secondary antibodies, and bulk quantities upon request. Our sourcing service helps diagnostic manufacturers and labs accelerate their R&D from concept to clinic.