Anti-eEF1G Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P26641

Core Product Specifications and Parameters

Biological Background: eEF1G Function and Localization

• Elongation factor 1-gamma (eEF1G), also known as EF1G, GIG35, or eEF-1B gamma, is a subunit of the eukaryotic elongation factor 1 complex essential for protein biosynthesis.
• eEF1G likely functions in anchoring the elongation factor complex to other cellular components, facilitating translational control.
• The protein is encoded by the EEF1G gene; alternative splicing may generate multiple isoforms (as suggested by UniProt keywords).
• Subcellular localization is not explicitly documented but is presumed cytoplasmic, associated with the protein synthesis machinery.
• Tissue expression: Highly expressed in pancreatic tumor tissue and, to a lesser extent, in normal kidney, intestine, pancreas, stomach, lung, brain, spleen, and liver.
• Post-translational modifications include acetylation, isopeptide bond formation, and ubiquitin-like conjugation, which may regulate protein stability and function.
• Keywords: 3D-structure, Direct protein sequencing, Proteomics identification, Reference proteome.

Experimental Guidance and Technical Tips

• The immunogen encompasses the N-terminal region (aa 1–123) of human eEF1G, which may be conserved across species, supporting the observed cross-reactivity with mouse and rat.
• This antibody has been reported for use in Western blotting (WB), immunohistochemistry on paraffin-embedded sections (IHC-P), immunofluorescence/immunocytochemistry (IF/ICC), and ELISA. Optimization of concentrations and protocols is recommended for each application.
• For WB, expect a band near 50 kDa. Validate using positive control lysates expressing eEF1G, such as pancreatic tumor cell lines.
• Verify specificity in knockout or knockdown systems if possible, particularly when studying closely related elongation factor subunits.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents for protein biosynthesis research. For scientists investigating eEF1G, CamelBio provides access to high-quality reagents and technical support to streamline your diagnostic development from concept to clinic.