Anti-EEF1E1 Polyclonal Antibody for WB, IF/ICC, ELISA - O43324

Core Product Specifications and Parameters

Biological Background: EEF1E1 Function and Localization

• Eukaryotic translation elongation factor 1 epsilon-1 (EEF1E1) is also known as aminoacyl tRNA synthetase complex-interacting multifunctional protein 3 (AIMP3) and elongation factor p18.
• It serves as an auxiliary component of the multisynthetase complex, which plays a role in protein biosynthesis.
• The protein functions as a positive modulator of the ATM-mediated DNA damage response.
• Subcellular localization is predominantly cytoplasmic and cytosolic, with additional presence in the nucleus.
• EEF1E1 expression is frequently down-regulated in various cancer tissues, suggesting a potential role in tumor suppression.
• Post-translational modifications include acetylation, and alternative splicing produces multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning the N-terminal region (amino acids 1-100) of human EEF1E1, which may contribute to high specificity.
• For Western blotting, a band around 20 kDa is expected; consider using a positive control such as lysates from human, mouse, or rat cell lines.
• In immunofluorescence/immunocytochemistry, permeabilization is recommended due to cytoplasmic localization; optimize fixation conditions for your cell type.
• When evaluating cancer tissue samples, be aware that EEF1E1 may be down-regulated; including a loading control can facilitate interpretation.
• For ELISA, perform a titration series to determine optimal coating antigen and antibody concentrations.
• This polyclonal antibody may detect multiple isoforms due to alternative splicing; confirm band patterns in relevant experimental contexts.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive sourcing solution for diagnostic manufacturers and researchers, providing high-quality IVD raw materials like this anti-EEF1E1 polyclonal antibody. Our portfolio supports DNA damage and cancer research applications with validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. Whether you need standard catalog items or rare target sourcing, CamelBio streamlines your procurement from concept to clinic.