Anti-EEF1D Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P29692

Core Product Specifications and Parameters

Biological Background: Elongation Factor 1-Delta Function and Localization

• EEF1D encodes elongation factor 1-delta, a subunit of the elongation factor-1 complex that also includes EF-1-alpha, EF-1-beta, and EF-1-gamma.
• EF-1-delta, together with EF-1-beta, catalyzes the exchange of GDP for GTP on EF-1-alpha, regenerating the active form of EF-1-alpha required for aminoacyl-tRNA delivery to the ribosome during protein synthesis.
• Beyond its canonical role in translation elongation, EEF1D functions in transcriptional regulation by associating with heat shock transcription factors and binding directly to heat shock promoter elements (HSE), thereby modulating heat-shock gene expression.
• The protein localizes predominantly to the nucleus, consistent with its DNA-binding and transcription regulatory activities.
• Alternative splicing produces multiple isoforms, including isoform 2 which shows tissue-specific expression in brain, cerebellum, and testis.
• Post-translational modifications include acetylation and phosphorylation, as identified by proteomics studies.
• Mutations in EEF1D have been linked to intellectual disability, marking it as a clinically relevant gene.

Experimental Guidance and Technical Tips

• The immunogen corresponds to residues 1-160 of human EEF1D (NP_001123529.1); the resulting polyclonal antibody may recognize multiple isoforms and is likely to detect the N-terminal region.
• For western blot (WB), a band at approximately 31 kDa is expected; consider using a positive control such as HeLa or HEK293 cell lysates.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), nuclear localization is anticipated; optimize antigen retrieval and blocking conditions for best results.
• This antibody has been reported for immunoprecipitation (IP); ensure appropriate negative controls (e.g., IgG isotype) to distinguish specific interactions.
• As a polyclonal antibody, lot-to-lot variability can occur; we recommend validating each new lot in your specific assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers a streamlined supply channel for high-quality IVD raw materials. This EEF1D antibody supports studies on protein synthesis and stress response pathways. Beyond single antibodies, we provide validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets to accelerate your assay development.