Anti-EEF1A2 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q05639

Core Product Specifications and Parameters

Biological Background: EEF1A2 Function and Localization

• EEF1A2 encodes elongation factor 1-alpha 2, a tissue-specific isoform of the alpha subunit of the elongation factor-1 complex, also known as eukaryotic elongation factor 1 A-2 (eEF1A-2) or statin-S1.
• It functions as a translation elongation factor, catalyzing the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein synthesis, followed by peptide bond formation.
• Unlike the ubiquitously expressed EEF1A1, EEF1A2 exhibits highly specific expression in brain, heart, and skeletal muscle.
• The protein localizes to the endoplasmic reticulum membrane, suggesting a role in co-translational processes.
• Post-translational modifications include acetylation, methylation, and phosphorylation, which may regulate its activity.
• Keywords associated with EEF1A2 include disease variants linked to autism spectrum disorder, epilepsy, and intellectual disability, indicating its neurological importance.
• It is a GTP-binding protein with hydrolase activity, requiring magnesium as a cofactor for nucleotide binding.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 184-463) targets the C-terminal region, potentially enhancing isoform specificity by avoiding the highly conserved N-terminal GTP-binding domain.
• As a polyclonal antibody, consider lot-to-lot consistency; validate performance in your specific assay context.
• For Western blot, expect a band near 50 kDa; use appropriate positive controls (e.g., brain, heart, or skeletal muscle lysates).
• In IHC-P, assess antigen retrieval conditions due to the endoplasmic reticulum membrane localization.
• For IF/ICC, fixation and permeabilization may require optimization to detect membrane-associated targets.
• ELISA suitability suggests utility for quantitative detection; pair with validated reagents for best results.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This anti-EEF1A2 rabbit polyclonal antibody supports translational research in protein synthesis mechanisms and neurological disease biomarker development, complementing our portfolio of high-quality immunoreagents for clinical assay construction.