Anti-EEF1A2 Polyclonal Antibody for WB and ELISA - Q05639

Core Product Specifications and Parameters

Biological Background: EEF1A2 Function and Localization

• EEF1A2 encodes elongation factor 1-alpha 2, also known as eukaryotic elongation factor 1 A-2 (eEF1A-2) or statin-S1, a member of the GTP-binding elongation factor family.
• It catalyzes the GTP-dependent binding of aminoacyl-tRNA to the ribosomal A-site during protein synthesis elongation, facilitating peptide bond formation (By similarity).
• EEF1A2 is a paralog of the ubiquitously expressed EEF1A1, but shows tissue-specific expression predominantly in brain, heart, and skeletal muscle.
• The protein localizes to the endoplasmic reticulum membrane, consistent with its role in cotranslational protein processing.
• Post-translational modifications include acetylation, methylation, and phosphorylation, which may regulate its activity; it also binds GTP and magnesium as cofactors.
• Mutations in EEF1A2 are associated with autosomal dominant neurological disorders, including epilepsy, intellectual disability, and autism spectrum disorder.
• It contains conserved GTP-binding domains and undergoes conformational changes upon GTP hydrolysis to release aminoacyl-tRNA, ensuring translation fidelity.

Experimental Guidance and Technical Tips

• For Western blot (WB), validate the expected band at ~50 kDa using lysates from human, mouse, or rat tissues; enrichment from brain, heart, or skeletal muscle is recommended due to tissue-specific expression.
• In ELISA, consider coating with recombinant EEF1A2 protein or a specific peptide; optimize antibody dilution and include appropriate controls.
• Use positive and negative controls (e.g., EEF1A2-overexpressing or knockdown cell lines) to confirm specificity.
• The immunogen (aa 184–463) covers the central domain; cross-reactivity with EEF1A1 should be assessed if necessary, as the two isoforms share high sequence identity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research laboratories with high-quality reagents and technical expertise. This EEF1A2 polyclonal antibody exemplifies our capability to provide validated antibodies for translational research and neurological disease biomarker development. From optimized monoclonal/polyclonal antibodies and validated antibody pairs to bulk ancillary reagents and rare target sourcing, CamelBio bridges the gap from concept to clinic with reliable supply solutions.