Anti-eEF1A1 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P68104

Core Product Specifications and Parameters

Biological Background: eEF1A1 Function and Localization

• eEF1A1 (Elongation factor 1-alpha 1) is a ubiquitously expressed protein that belongs to the GTP-binding elongation factor family. It is also known as EF-Tu, eukaryotic elongation factor 1 A-1 (eEF1A-1), and leukocyte receptor cluster member 7.
• As a core component of the translational machinery, eEF1A1 catalyzes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein synthesis elongation, facilitating codon-anticodon recognition and peptide bond formation. Related references: PMID:26593721, PMID:26651998, PMID:36123449
• eEF1A1 also plays a non-canonical role in transcriptional regulation, positively modulating IFNG expression in T-helper 1 cells by forming a complex with TXK and PARP1 at the IFNG promoter. Related references: PMID:17177976
• The protein is involved in cytoskeleton organization by promoting actin bundling.
• Subcellularly, eEF1A1 localizes primarily to the cytoplasm, but is also found in the nucleus, nucleolus, and at the cell membrane.
• During SARS-CoV-2 infection, eEF1A1 is required for the translation of viral proteins and efficient viral replication.
• Post-translational modifications of eEF1A1 include acetylation, methylation, phosphorylation, and ubiquitination, as identified through proteomics approaches.
• The calculated molecular weight of eEF1A1 is approximately 50.1 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 300-400 of human eEF1A1, a region that is likely to be well-conserved across species, underpinning the antibody's cross-reactivity with mouse and rat.
• For Western blotting, a band at approximately 50 kDa is expected; consider using appropriate protein extraction and loading controls.
• The antibody is suitable for IHC-P and IF/ICC; optimization of fixation and antigen retrieval conditions may be required for different tissue types.
• For ELISA applications, titrate the antibody to determine the optimal working concentration for your specific assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers a comprehensive sourcing solution for IVD raw materials, covering every stage from concept to clinic. This anti-eEF1A1 monoclonal antibody is part of our extensive portfolio for translation and protein synthesis research, which includes validated antibody pairs and optimized reagents. For your projects requiring bulk quantities or rare target materials, CamelBio can source and supply tailored raw materials to accelerate your assay development.