Anti-EEA1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q15075

Core Product Specifications and Parameters

Biological Background: EEA1 Function and Localization

• EEA1 (Early endosome antigen 1), also designated endosome-associated protein p162 or ZFYVE2, is a ~162 kDa protein encoded by the EEA1 gene in humans.
• It functions as a tethering factor by binding to phosphatidylinositol 3-phosphate (PtdIns(3)P) on endosomal membranes, facilitating vesicle docking and fusion during early endosome trafficking.
• The protein localizes predominantly to the cytoplasmic face of early endosome membranes and is also present in the cytosol, consistent with its role in membrane dynamics.
• EEA1 contains an N-terminal C2H2 zinc finger, extensive coiled-coil domains, and a C-terminal FYVE zinc finger domain that directly mediates PtdIns(3)P binding.
• Post-translational modifications include phosphorylation, and the protein is subject to proteomics identification, indicating broad expression across tissues.
• Keywords: 3D-structure, Coiled coil, Cytoplasm, Direct protein sequencing, Endosome, Membrane, Metal-binding, Phosphoprotein, Proteomics identification, Reference proteome, Zinc, Zinc-finger.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1182–1411 of human EEA1, covering the C-terminal region that includes the FYVE domain. This may contribute to specific recognition of the full-length protein.
• For Western blot, a major band near 162 kDa is expected. Use low-percentage SDS-PAGE gels and extended transfer times to improve detection of this high molecular weight target.
• In IHC-P experiments, antigen retrieval conditions should be optimized for each tissue type, and appropriate positive and negative controls are recommended.
• For ELISA applications, consider using the antibody as a capture or detection reagent after validation with recombinant EEA1 or cell lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with comprehensive IVD raw material supply, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. For targets like EEA1, central to endosomal trafficking and intracellular signaling studies, we offer reliable polyclonal antibodies alongside tailored sourcing solutions for rare or challenging targets, supporting your entire development workflow from concept to clinic.