Anti-EDN1 Polyclonal Antibody (KO Validated) for WB, IF/ICC, ELISA - P05305

Core Product Specifications and Parameters

Biological Background: Endothelin-1 Function and Localization

• Endothelin-1 is encoded by the EDN1 gene and produced as a preproprotein (PPET1) that undergoes proteolytic processing to yield the mature 21-amino acid vasoactive peptide.
• It belongs to the endothelin family, which are potent vasoconstrictors primarily secreted by endothelial cells.
• Endothelin-1 acts as a ligand for G protein-coupled receptors EDNRA and EDNRB, leading to activation of PTK2B, BCAR1, BCAR3, and GTPases RAP1 and RHOA in glomerular mesangial cells. Related references: PMID:19086031
• It also binds to the DEAR/FBXW7-AS1 receptor. Related references: PMID:17446437
• By similarity, endothelin-1 promotes mesenteric arterial wall remodeling through ROCK signaling, leading to NFATC3 nuclear translocation and upregulation of ACTA2, a marker of smooth muscle hypertrophy.
• Subcellular localization: Secreted into the extracellular space.
• Tissue specificity: Expressed in lung, placental stem villi vessels, and cultured placental vascular smooth muscle cells.
• Keywords: Vasoactive, vasoconstrictor, disulfide bond, secreted, signal peptide, and disease variant.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 18-212 of human EDN1, covering the preproprotein region. The antibody may recognize both precursor and mature forms; consider this when analyzing bands in Western blot.
• Predicted molecular weight of the preproprotein is 24 kDa; mature endothelin-1 is approximately 2.5 kDa. Optimize gel percentage and transfer conditions for small peptides if needed.
• For IF/ICC, validate permeabilization and fixation conditions, as endothelin-1 is secreted; consider co-staining with organelle markers.
• When using for ELISA, pair with an appropriate detection antibody and validate in the relevant sample matrix (e.g., serum, plasma) to avoid interference.
• Cross-reactivity with mouse and rat has been reported; verify specificity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, research labs, and institutes with one-stop access to IVD raw materials, technical services, and consulting, covering every stage from concept to clinic. This anti-EDN1 polyclonal antibody targets a key vasoconstrictor peptide involved in cardiovascular and renal research, making it a valuable tool for the development of immunoassays and research reagents. Beyond this product, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to support your IVD development needs.