Anti-EDEM2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9BV94

Core Product Specifications and Parameters

Biological Background: EDEM2 Function and Localization

• EDEM2 (C20orf31, C20orf49, bA4204.1) is an ER degradation-enhancing alpha-mannosidase-like protein 2.
• It plays a key role in the endoplasmic reticulum-associated degradation (ERAD) pathway, recognizing and targeting misfolded glycoproteins for proteasomal degradation in an N-glycan-dependent manner. Related references: PMID:15537790 PMID:25092655
• EDEM2 is proposed to initiate ERAD by catalyzing the first mannose trimming step of substrate glycans, converting Man9GlcNAc2 to Man8GlcNAc2. Related references: PMID:25092655
• It likely binds to exposed hydrophobic patches on misfolded proteins, facilitating their retrotranslocation and degradation.
• The protein localizes to the lumen of the endoplasmic reticulum.
• EDEM2 is ubiquitously expressed across human tissues, with strongest expression in small intestine and peripheral blood leukocytes and lowest in brain and skeletal muscle.
• It is subject to alternative splicing and participates in the unfolded protein response.
• The predicted molecular weight of human EDEM2 is approximately 65 kDa.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant fragment covering amino acids 399–578 of human EDEM2, a region that may confer target specificity; consider verifying with your own controls.
• For Western blot, a band around 65 kDa is expected; using cell lines such as HeLa or HEK293 as positive controls is advisable, but validation in your specific sample system is recommended.
• In immunocytochemistry (IF/ICC), fixation and permeabilization conditions should be optimized for ER luminal proteins; initial testing with known EDEM2-expressing cells can help establish the protocol.
• ELISA applications can be developed using recombinant EDEM2 protein or cell-based assays; titrate the antibody to determine the optimal working concentration.
• The antibody cross-reacts with mouse EDEM2, supporting its use in mouse model studies; however, always confirm reactivity with appropriate mouse tissue lysates.
• As a polyclonal antibody, lot-to-lot variability may occur; include positive and negative controls in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a streamlined sourcing solution for high-quality IVD raw materials. With this anti-EDEM2 antibody, we support investigations into ERAD and glycoprotein quality control, and we can supply validated antibody pairs, bulk ancillary reagents, and rare-target raw materials to accelerate your project from concept to clinic.