Anti-EDC4 Rabbit Polyclonal Antibody for WB, ELISA - Q6P2E9

Core Product Specifications and Parameters

Biological Background: EDC4 Function and Localization

• EDC4 (Enhancer of mRNA-decapping protein 4), also known as Ge-1, Hedls, or RCD-8, is a key component of the mRNA decapping machinery.
• It functions as a scaffold in a complex with decapping enzymes DCP1A and DCP2, promoting their interaction and enhancing DCP2 catalytic activity in vitro.
• EDC4 plays a role in the degradation of ARE-containing mRNAs through the decapping pathway.
• The protein contains WD repeats and a coiled-coil domain, facilitating protein-protein interactions.
• Subcellularly, EDC4 localizes to the cytoplasm and nucleus, with enrichment in processing bodies (P-bodies), cytoplasmic foci involved in mRNA decay.
• Post-translational modifications include acetylation and phosphorylation, as identified by proteomic studies.
• Alternative splicing generates multiple isoforms of EDC4.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (aa 800-1100), which may detect multiple isoforms; verify specificity in the relevant cell type.
• For Western blot, a 152 kDa band is expected; use a low-percentage SDS-PAGE gel and transfer conditions optimized for high-molecular-weight proteins.
• Cross-reactivity is reported for human and mouse; verify reactivity in other species before use.
• For ELISA applications, test the antibody in both direct and sandwich formats to determine optimal performance.
• Consider using this antibody to study P-body dynamics or mRNA decay pathways in cell-based assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, including validated antibodies like this EDC4 polyclonal antibody for mRNA decay research. Beyond individual reagents, we offer optimized monoclonal/polyclonal pairs, bulk ancillary reagents, and rare target sourcing to accelerate your assay development from concept to clinic. Contact us for customized procurement solutions.