Anti-EDC3 Rabbit Polyclonal Antibody for WB - Q96F86

Core Product Specifications and Parameters

Biological Background: EDC3 Function and Localization

• EDC3 (Enhancer of mRNA-decapping protein 3), also known as LSM16 homolog, YjeF_N2, or YJDC, is an RNA-binding protein that specifically binds single-stranded RNA.
• It functions as a positive regulator of mRNA decapping, a critical step in the 5'-to-3' mRNA degradation pathway, thereby modulating post-transcriptional gene expression.
• EDC3 localizes to cytoplasmic processing bodies (P-bodies), which are discrete cytoplasmic foci involved in mRNA storage and decay.
• At the protein level, EDC3 is prominently expressed in ovarian theca and granulosa cells, as well as in testicular spermatids and Sertoli cells, suggesting roles in spermiogenesis and oogenesis.
• Additionally, expression has been detected in brain and mammary gland tissues, indicating broader functional relevance.
• The protein belongs to the LSM16 family and contains a YjeF N-terminal domain, characteristic of RNA-processing factors.
• Post-translational modifications include phosphorylation, and variants in EDC3 have been associated with disease phenotypes, notably intellectual disability (keyword evidence).

Experimental Guidance and Technical Tips

• The immunogen, corresponding to amino acids 159–508 of human EDC3, covers a conserved region; cross-reactivity with mouse and rat is reported—validate sequence homology between species for your specific experimental model.
• For Western blotting, expect a band around 56 kDa (theoretical molecular weight 56,078 Da from UniProt); use lysates from tissues with high EDC3 expression (e.g., testis, ovary, brain) as positive controls.
• In ELISA, coating with recombinant EDC3 protein or the immunogen fragment is recommended; optimize antibody dilution and incubation conditions to minimize background.
• Although validated for WB and ELISA, the antibody may be suitable for immunoprecipitation or immunofluorescence; however, empirical validation is required for non-validated applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop IVD raw-material sourcing partner, supporting diagnostic manufacturers, laboratories, and research institutions from early development through clinical application. For projects targeting mRNA decapping, post-transcriptional regulation, or EDC3-related pathways, CamelBio supplies validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. Our team also facilitates custom sourcing of rare-target raw materials, ensuring a streamlined supply chain for your assay development needs.