Anti-EDARADD Rabbit Polyclonal Antibody for WB, ELISA - Q8WWZ3

Core Product Specifications and Parameters

Biological Background: EDARADD Function and Localization

• EDARADD (Ectodysplasin-A receptor-associated adapter protein) is also known as EDAR-associated death domain protein, protein crinkled homolog, and by the aliases CR, ED3, EDA3, ECTD11A, and ECTD11B.
• It functions as an adapter protein that interacts with the death domain of EDAR (ectodysplasin-A receptor), coupling the receptor to downstream signaling cascades.
• The primary signaling pathway mediated by EDARADD involves activation of NF-kappa-B, which is crucial for the morphogenesis of ectodermal organs such as hair, teeth, and sweat glands.
• Subcellularly, EDARADD is located in the cytoplasm, where it relays signals from the cell surface receptor.
• Expression of EDARADD is detected in adult pancreas, placenta, and fetal skin, with lower levels in lung, thymus, prostate, and testis, indicating a role in both developmental and adult tissues.
• The protein undergoes alternative splicing, generating multiple isoforms that may have tissue-specific functions, as annotated in the UniProt record.
• Mutations in the EDARADD gene are linked to ectodermal dysplasia syndromes, including ECTD11A and ECTD11B, underscoring its developmental importance.
• Additional keywords from its UniProt entry include developmental protein, differentiation, and reference proteome, highlighting its essential role in epidermal development.

Experimental Guidance and Technical Tips

• The immunogen used to generate this polyclonal antibody comprises a recombinant fusion protein corresponding to amino acids 1-215 of human EDARADD (NP_665860.2). This near full-length antigen is expected to elicit antibodies against multiple epitopes, potentially enhancing detection sensitivity.
• Given its validation in WB and ELISA applications, users may consider optimizing antibody dilutions empirically; typical starting dilutions for polyclonal antibodies in WB range from 1:500 to 1:2000, but specific optimization for the target protein and sample type is recommended.
• Cross-reactivity with mouse and rat EDARADD is indicated, allowing potential use across these species; however, sequence homology should be verified for the intended epitope regions, and experimental conditions should be validated in the relevant sample system.
• For ELISA, pair this detection antibody with a suitable capture antibody or antigen coating to develop sandwich or indirect ELISA protocols; controls and standard curves are essential for quantitative analysis.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers IVD manufacturers and research laboratories a comprehensive portfolio of high-quality raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. For projects targeting ectodermal dysplasia-related pathways or NF-kB signaling, we provide tailored sourcing of rare and niche raw materials, backed by technical consulting from concept to clinical application.