Anti-EDARADD Rabbit Polyclonal Antibody for WB and ELISA - Q8WWZ3

Core Product Specifications and Parameters

Biological Background: EDARADD Function and Localization

• EDARADD (Ectodysplasin-A receptor-associated adapter protein), also known as EDAR-associated death domain protein or crinkled homolog, is encoded by the EDARADD gene and undergoes alternative splicing.
• It functions as an adapter protein that directly interacts with the death domain of the ectodysplasin-A receptor (EDAR), coupling it to the downstream EDA signaling pathway.
• This protein plays a critical role in the morphogenesis of ectodermal organs, mediating the activation of the NF-kappa-B transcription factor.
• Subcellularly, EDARADD localizes to the cytoplasm where it participates in signal transduction from the plasma membrane receptor.
• Tissue expression analysis shows EDARADD is detected at high levels in adult pancreas, placenta, and fetal skin, with lower expression in lung, thymus, prostate, and testis.
• Mutations in EDARADD are associated with ectodermal dysplasia syndromes, highlighting its importance in developmental processes and differentiation.
• The protein is classified under keywords such as developmental protein, differentiation, and disease variant, underscoring its biological significance.

Experimental Guidance and Technical Tips

• For western blotting, a band at approximately 25 kDa is expected; consider validating with lysates from human pancreatic or placental tissues where EDARADD is expressed.
• When using ELISA, titrate the antibody with recombinant EDARADD protein to establish a standard curve and optimize detection conditions.
• As a polyclonal antibody, batch-to-batch consistency should be verified, and proper controls (e.g., negative control lysates or peptide blocking) are recommended to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research teams with one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For targets like EDARADD involved in developmental biology and ectodermal dysplasia research, we can source rare or custom raw materials to streamline your assay development. Our supply chain support spans from concept to clinic, helping you accelerate project timelines with reliable, quality-controlled reagents.