Anti-ECSIT Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9BQ95

Core Product Specifications and Parameters

Biological Background: ECSIT Function and Localization

• ECSIT plays a key role in NF-kB activation by assembling a signal complex with TRAF6 and TAK1/MAP3K7, which activates IKKs. Related references: PMID:25355951, PMID:31281713
• Once ubiquitinated, ECSIT binds RELA and NFKB1 and translocates to the nucleus to drive NF-kB-dependent transcription. Related references: PMID:25355951
• ECSIT bridges RIG-I and MDA5 pattern recognition receptors to the MAVS complex at mitochondria, supporting innate antiviral immune signaling. Related references: PMID:25228397
• As a component of the MCIA complex, ECSIT is essential for the assembly of mitochondrial complex I.
• ECSIT is involved in the BMP signaling pathway and is required for normal embryonic development (by similarity).
• Subcellularly, ECSIT localizes to the cytoplasm, nucleus, and mitochondrion.
• Keywords: alternative splicing, cytoplasm, nucleus, mitochondrion, innate immunity, Ubl conjugation (ubiquitination), and transit peptide.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 50-296 of human ECSIT, a region that may be conserved across human, mouse, and rat, consistent with the reported cross-reactivity.
• For Western blot, a band around 49 kDa (predicted molecular weight) is expected; consider using positive controls such as HeLa or HEK293 cell lysates.
• In IF/ICC applications, ECSIT exhibits cytoplasmic, nuclear, and mitochondrial staining; co-localization experiments with appropriate markers may help confirm specificity.
• Because ECSIT functions in multiple signaling complexes, experimental conditions (e.g., stimulation) may influence detection; consider piloting conditions for your specific model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw materials, offering validated antibody reagents like this anti-ECSIT polyclonal antibody, which targets a key adaptor in innate immunity and mitochondrial function. From monoclonal/polyclonal antibodies to antigen-grade recombinant proteins and ancillary reagents, we streamline your procurement process—whether you need bulk quantities or support for rare targets. Let our expertise in immunodiagnostic development accelerate your projects from concept to clinic.