Anti-ECM1 Polyclonal Antibody for WB, IF/ICC, ELISA - Q16610

Core Product Specifications and Parameters

Biological Background: ECM1 Function and Localization

• ECM1 (Extracellular matrix protein 1), also known as secretory component p85, is a secreted glycoprotein encoded by the ECM1 gene.
• It acts as a negative regulator of endochondral bone formation by inhibiting bone mineralization.
• ECM1 stimulates endothelial cell proliferation and promotes angiogenesis, playing a role in vascular development.
• The protein inhibits the proteolytic activity of MMP9, potentially modulating extracellular matrix remodeling.
• Subcellularly, ECM1 is localized to the extracellular space and extracellular matrix, consistent with its secreted nature.
• Tissue expression is elevated in breast cancer tissues, with minimal expression in normal breast; in skin, it is widely expressed in the dermis but low in the epidermis.
• Keywords from UniProt highlight its involvement in alternative splicing, angiogenesis, biomineralization, and its role as a glycoprotein associated with disease variants.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 280–405 of human ECM1; consider verifying cross-reactivity with mouse model systems if applying to mouse samples.
• For Western blotting, ECM1 has a predicted molecular weight of 61kDa; however, actual migration may shift due to glycosylation—validate with appropriate controls in tissue lysates from breast cancer or skin.
• In immunofluorescence/immunocytochemistry, since ECM1 is secreted, optimize permeabilization and fixation conditions to detect both intracellular and extracellular matrix-associated pools.
• For ELISA applications, consider using this antibody to capture ECM1 in cell culture supernatants or biological fluids, pairing with a detection antibody for sandwich ELISA.
• It is recommended to validate the antibody's performance in the specific sample system and application prior to use in diagnostic or analytical workflows.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research labs a streamlined, one-stop sourcing solution for high-quality IVD raw materials. For targets like ECM1 involved in bone disorders, angiogenesis, and oncology, we supply validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. Our expertise also extends to sourcing rare-target raw materials, supporting your project from concept to clinic with reliable procurement and technical consultation.