Anti-ECI2 Rabbit Polyclonal Antibody for WB and ELISA - O75521

Core Product Specifications and Parameters

Biological Background: ECI2 Function and Localization

• Enoyl-CoA delta isomerase 2 (ECI2), also known as DRS-1, PECI, or HCA88, is a mitochondrial and peroxisomal enzyme that catalyzes the isomerization of 3-cis and 3-trans double bonds in enoyl-CoA species to the 2-trans form, with a preference for 3-trans substrates.
• The protein is encoded by the ECI2 gene (GeneID: 10455) in humans and is essential for the beta-oxidation of unsaturated fatty acids in both mitochondria and peroxisomes.
• ECI2 is abundant in heart, skeletal muscle, and liver, and is also expressed in CD34+ T-cells and CD34+ bone marrow cells, reflecting its role in fatty acid metabolism in high-energy-demand tissues.
• Subcellularly, ECI2 localizes to the mitochondrial matrix and the peroxisome matrix, where it functions as a key isomerase in the degradation of unsaturated fatty acids.
• Post-translational modifications include acetylation and phosphorylation, as identified by proteomics approaches; the protein also contains a transit peptide for organellar targeting.
• Alternative splicing generates multiple isoforms, potentially regulating its activity or localization across tissues.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human ECI2 protein (amino acids 1-364), which suggests the antibody likely recognizes multiple isoforms and is suitable for detecting total ECI2 levels.
• For Western blot, a band around 44kDa is expected; using appropriate tissue lysates from heart, skeletal muscle, or liver (mouse, rat, or human) may enhance detection.
• For ELISA, consider optimizing coating antigen and antibody dilution to achieve optimal signal-to-noise ratio; note that the antibody is a polyclonal, which may provide robust binding but could exhibit lot-to-lot variability.
• If cross-reactivity is critical, validate in the relevant species (mouse, rat) as indicated by the manufacturer, but verify in your own experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your strategic partner for sourcing high-quality IVD raw materials, offering one-stop access to validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This ECI2 antibody supports research in fatty acid metabolism, peroxisomal disorders, and cancer biomarker studies. Trust CamelBio to streamline your procurement from concept to clinic with rigorous supply-chain management.