Anti-ECI1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P42126

Core Product Specifications and Parameters

Biological Background: ECI1 Function and Localization

• ECI1 (Enoyl-CoA delta isomerase 1, mitochondrial), also known as DCI or dodecenoyl-CoA isomerase, is a mitochondrial enzyme encoded by the ECI1 gene.
• It functions as an auxiliary enzyme in the beta-oxidation of mono- and polyunsaturated fatty acids, catalyzing the isomerization of 3-cis and 3-trans double bonds to the 2-trans form to allow re-entry into the beta-oxidation cycle. Related references: PMID:26474213, PMID:7818490
• Together with 2,4-dienoyl-CoA reductase (DECR) and Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase (ECH1), ECI1 ensures efficient degradation of unsaturated fatty acids. Related references: PMID:26474213
• Subcellularly localized to the mitochondrial matrix, consistent with its role in fatty acid metabolism.
• Tissue expression is notably high in liver, reflecting the organ's central role in fatty acid oxidation.
• The protein is subject to acetylation and alternative splicing, and it contains a transit peptide for mitochondrial import.
• The molecular weight is approximately 33 kDa (32816 Da according to UniProt, consistent with the observed 33kDa band).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 63–302 of human ECI1, covering the mature mitochondrial form; consider this when interpreting band sizes on western blots.
• For WB, load appropriate positive control lysates (e.g., human liver) and validate the ~33 kDa band; include a mitochondrial fraction if needed.
• For IHC-P and IF/ICC, optimize antigen retrieval and use human liver sections as a positive control given the high hepatic expression.
• The antibody cross-reacts with mouse, allowing use in rodent models, but validate in the relevant sample system as performance may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and laboratories with a comprehensive portfolio of IVD raw materials, including validated antibodies for metabolic and mitochondrial targets such as ECI1. We offer optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and tailored sourcing solutions to streamline your assay development. Partner with us to efficiently bridge the gap from concept to clinic with reliable, high-quality raw materials.