Anti-ECH1 Rabbit Monoclonal Antibody (KD Validated) for WB, IHC-P, IF/ICC, ELISA - Q13011

Core Product Specifications and Parameters

Biological Background: ECH1 Function and Localization

• ECH1 encodes delta(3,5)-delta(2,4)-dienoyl-CoA isomerase, a mitochondrial and peroxisomal enzyme involved in fatty acid metabolism.
• It catalyzes the isomerization of 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, a key step in the beta-oxidation of unsaturated fatty acids. Related references: PMID:26474213
• Acts together with 2,4-dienoyl-CoA reductase (DECR) and enoyl-CoA delta isomerase 1 (ECI1) to enable re-entry of enoyl-CoA intermediates into the beta-oxidation cycle. Related references: PMID:26474213
• Localizes to both mitochondria and peroxisomes, indicating a role in the metabolism of fatty acids across these organelles.
• Post-translational modifications include acetylation and phosphorylation, which may regulate its activity (based on UniProt keywords).
• The theoretical molecular weight of human ECH1 is 35.8 kDa (UniProt Q13011); this antibody detects a band at approximately 36 kDa in WB applications.
• Known by the alias HPXEL.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 50-322 of human ECH1, likely targeting the mature protein after cleavage of the mitochondrial transit peptide.
• For western blot, the expected band is around 36 kDa; consider using appropriate loading controls.
• Detects endogenous ECH1 in human, mouse, and rat samples; cross-reactivity with other species should be experimentally validated.
• Suitable for IHC-P and IF/ICC; optimize antigen retrieval and fixation protocols for best results.
• KD-validated by the manufacturer, confirming target specificity; perform additional validation for non-standard applications.

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