Anti-ECD Rabbit Polyclonal Antibody for WB and ELISA - O95905

Core Product Specifications and Parameters

Biological Background: ECD Function and Localization

• Protein ecdysoneless homolog (ECD), also known as hSGT1 or GCR2, is a regulator of p53/TP53 stability and function. It inhibits MDM2-mediated degradation of p53/TP53, possibly cooperating with TXNIP. Related references: PMID:16849563, PMID:23880345
• ECD may act as a transcriptional regulator with intrinsic transactivation activity enhanced by EP300. It is potentially required for the expression of glycolytic genes. Related references: PMID:19919181, PMID:9928932
• Involved in cell cycle progression, ECD is proposed to disrupt Rb-E2F binding, leading to transcriptional activation of E2F proteins. This function may depend on its association with the R2TP complex via RUVBL1. Related references: PMID:19640839, PMID:26711270
• ECD plays a role in pre-mRNA splicing regulation and participates in mRNA nuclear export together with DDX39A. Related references: PMID:24722212, PMID:33941617
• The protein localizes to both the cytoplasm and nucleus, consistent with its roles in transcription, splicing, and mRNA export.
• ECD is highly expressed in muscle and heart, and over-expressed in pancreatic and breast cancers, suggesting potential relevance in oncology research.
• Post-translational modifications include phosphorylation, and the gene undergoes alternative splicing, indicating regulatory complexity.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to amino acids 435-644 of the human ECD protein, which covers the C-terminal region. This region may contain epitopes conserved across human, mouse, and rat, supporting the reported cross-reactivity.
• For Western blotting (WB), the predicted molecular weight is approximately 73 kDa. It is advisable to verify band specificity using appropriate controls.
• For ELISA, consider using the recombinant protein as a standard and optimize coating conditions.
• Since ECD localizes to both cytoplasm and nucleus, subcellular fractionation may enhance detection in WB.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated polyclonal and monoclonal antibodies, antibody pairs, and bulk ancillary reagents. Our ECD antibody supports investigations into p53 regulation, cell cycle control, and cancer biology. For your specific workflow needs, we offer tailored sourcing solutions for rare or custom targets from discovery to clinical application.