Anti-EBP1/PA2G4 Polyclonal Antibody for WB, IHC-P, ELISA - Q9UQ80

Core Product Specifications and Parameters

Biological Background: EBP1/PA2G4 Function and Localization

• EBP1, encoded by the PA2G4 gene, is also known as proliferation-associated protein 2G4, ErbB3-binding protein 1, and cell cycle protein p38-2G4 homolog (hG4-1). It exists as at least two isoforms generated by alternative splicing; isoform 1 suppresses apoptosis, while isoform 2 promotes cell differentiation.
• The protein plays a role in ERBB3-regulated signal transduction pathways, acting as a corepressor of the androgen receptor (AR) and inhibiting transcription of certain E2F1-regulated promoters, likely through recruitment of histone acetylase (HAT) activity.
• EBP1 binds RNA and associates with mature rRNAs (28S, 18S, 5.8S) and rRNA precursors, suggesting involvement in ribosomal RNA processing and ribosome assembly. It may also mediate cap-independent translation of viral IRES elements.
• Subcellular localization includes cytoplasm, nucleus, and nucleolus, consistent with its roles in signal transduction, transcriptional regulation, and ribosome biogenesis.
• The protein is regulated by phosphorylation, acetylation, and ubiquitination, as indicated by keywords "Phosphoprotein", "Acetylation", and "Ubl conjugation". A 3D structure is available in the PDB.
• Isoform 1 is strongly expressed in cancer cells, while isoform 2 is undetectable at the protein level in these cells. Both isoforms are widely expressed in normal tissues, including heart, brain, lung, pancreas, skeletal muscle, kidney, placenta, and liver.

Experimental Guidance and Technical Tips

• As a polyclonal antibody, batch-to-batch consistency may vary; consider validating each new lot in your specific experimental setup.
• For western blot (WB), the expected molecular weight is approximately 44 kDa. Running appropriate positive controls (e.g., HeLa, HEK293 lysates) is recommended.
• In immunohistochemistry (IHC-P), antigen retrieval methods may be required for optimal staining. Titrate the antibody to determine the optimal working dilution for your tissue type.
• The antibody reacts with human, mouse, and rat samples; cross-reactivity with other species has not been determined.
• ELISA applications may be feasible; ensure careful optimization of coating antigens and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials and technical services, from concept to clinic. Our extensive catalog includes validated monoclonal and polyclonal antibodies, along with bulk ancillary reagents, to support your assay development. For research on ERBB3 signaling, cell cycle regulation, or ribosomal biogenesis, CamelBio is your reliable sourcing partner for high-quality reagents like this EBP1/PA2G4 antibody.