Anti-EBP Polyclonal Antibody for WB and ELISA - Q15125

Core Product Specifications and Parameters

Biological Background: EBP Function and Localization

• EBP (3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase) is a key enzyme in the post-lanosterol cholesterol biosynthesis pathway, catalyzing the conversion of Delta(8)-sterols to Delta(7)-isomers.
• As a component of the microsomal antiestrogen binding site (AEBS) complex, EBP associates with DHCR7 to form a cholesterol-5,6-epoxide hydrolase (ChEH) that hydrates cholesterol-5,6-epoxides into cholestane-3beta,5alpha,6beta-triol. Related references: PMID:15175332 PMID:20615952
• The protein resides in the endoplasmic reticulum membrane, nuclear envelope, and cytoplasmic vesicles, reflecting its roles in sterol metabolism and intracellular trafficking.
• Mutations in EBP are linked to human disorders such as X-linked dominant chondrodysplasia punctata (CDPX2), featuring dwarfism, cataracts, and ichthyosis.
• EBP is also known as emopamil-binding protein, highlighting its pharmacological relevance as a target of the anti-ischemic drug emopamil.
• The protein contains a transmembrane helix and exhibits dual isomerase and hydrolase activities, essential for maintaining cholesterol homeostasis.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the central region (aa 50-150) of human EBP; consider this when selecting epitope retrieval methods for immunohistochemistry.
• For Western blot, a band around 26 kDa is expected; validate in your sample system and adjust loading controls accordingly.
• ELISA performance may depend on the detection system; optimize coating and blocking conditions for best signal-to-noise.
• Cross-reactivity with mouse and rat has been reported; verify sequence homology in other species before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to IVD raw materials across the entire development pipeline. As a one-stop partner, we supply validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents for cholesterol metabolism and steroid biosynthesis targets. Whether you require rare-target sourcing or consistent lot-to-lot performance, CamelBio bridges your concept-to-clinic needs with reliable supply solutions.