Anti-EBNA1BP2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q99848

Core Product Specifications and Parameters

Biological Background: EBNA1BP2 (EBP2) Function and Localization

• EBNA1BP2 encodes probable rRNA-processing protein EBP2, also known as EBNA1-binding protein 2 or nucleolar protein p40, a 34852 Da protein essential for ribosome biogenesis.
• The protein is required for the processing of 27S pre-rRNA, a critical step in the maturation of ribosomal RNA, thereby supporting ribosome assembly.
• EBP2 localizes predominantly to the nucleolus within the nucleus, consistent with its role in rRNA processing.
• Expression of EBNA1BP2 is ubiquitous across human tissues, reflecting its fundamental housekeeping function in ribosome biogenesis.
• The protein contains coiled-coil domains and is subject to multiple post-translational modifications, including acetylation, phosphorylation, and ubiquitin-like conjugation (e.g., isopeptide bond formation), which may regulate its function or interactions.
• Structural studies (3D-structure) and proteomics identification have been reported, underlining its conserved role in nucleolar processes.
• As a ribosomal biogenesis factor, EBP2 is implicated in cell growth and proliferation, and its interactions with viral proteins (e.g., EBNA1) highlight its potential role in cellular stress responses.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1–306 of human EBNA1BP2, covering nearly the full-length protein; thus, the polyclonal antibody may recognize multiple epitopes, reducing signal loss from minor truncations.
• For Western blotting, expect a band around 35 kDa; validate in relevant human, mouse, or rat cell lysates, and consider using nucleolar enrichment protocols to enhance detection.
• In immunofluorescence/ICC, anticipate nucleolar staining; co-stain with a nucleolar marker to confirm specificity.
• For ELISA, the antibody’s reactivity with recombinant protein suggests suitability for direct detection; titration and optimization under assay-specific conditions are advised.
• Since this is a polyclonal antibody, lot-to-lot variation is possible; include appropriate controls and validate in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to a comprehensive portfolio of IVD raw materials, including validated antibodies, recombinant proteins, and ancillary reagents. The EBNA1BP2 (EBP2) target is central to ribosome biogenesis studies, and we offer this polyclonal antibody along with expert technical support to accelerate your assay development. With robust supply capabilities—from bulk orders to rare-target sourcing—CamelBio serves as your end-to-end procurement partner, simplifying the path from concept to clinic.