Anti-EBI3 Rabbit Monoclonal Antibody for WB, ELISA - Q14213

Core Product Specifications and Parameters

Biological Background: EBI3/IL-27 Subunit Beta Function and Localization

• EBI3, also known as Epstein-Barr virus-induced gene 3 protein, is the beta subunit of the heterodimeric cytokines IL-27 and IL-35.
• Paired with IL27 (p28) to form IL-27, which plays dual pro- and anti-inflammatory roles in innate immunity, regulating T-helper cell differentiation, T-cell proliferation, and cytotoxic T-cell activity.
• IL-27 synergizes with IL-12 to induce interferon-gamma production in naive CD4+ T-cells and drives clonal expansion of these cells.
• Exhibits antitumor activity and antiangiogenic effects by inducing antiangiogenic chemokines.
• Subcellular localization is primarily secreted, consistent with its function as a circulating cytokine.
• Post-translational modifications include glycosylation, and the protein contains fibronectin type-III domains.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 130-229, a region within the extracellular domain. This suggests the antibody may recognize a linear epitope, making it suitable for Western blotting under denaturing and reducing conditions.
• For WB, using a sample type known to express EBI3, such as immune cells or tissue lysates, is recommended. The predicted molecular weight is ~25 kDa, though glycosylation may cause a shift.
• In ELISA applications, consider pairing with a detection antibody that recognizes a distinct epitope. Validate specificity using recombinant EBI3 protein or biological samples.
• Cross-reactivity with mouse and rat is indicated; verify performance in the relevant species and application experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated sourcing partner for IVD raw materials, offering a comprehensive range of antibodies, antigen proteins, and ancillary reagents. This anti-EBI3 antibody supports research in cytokine signaling and immune regulation, and we can supply additional validated antibody pairs, bulk monoclonal/polyclonal antibodies, and custom sourcing for rare targets to streamline your diagnostic development.