Anti-EBF3 Polyclonal Antibody for WB, ELISA - Q9H4W6

Core Product Specifications and Parameters

Biological Background: EBF3 Function and Localization

• EBF3, also known as Transcription factor COE3, Olf-1/EBF-like 2, and O/E-2, is a member of the early B-cell factor (EBF) family of transcription factors.
• Acts as a transcriptional activator, regulating gene expression by binding to specific DNA sequences.

Related references: PMID:28017370 PMID:28017372 PMID:28017373

• Recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3' for DNA binding.
• Localizes primarily to the nucleus, consistent with its role in transcriptional regulation.
• Predominantly expressed in the brain, where it plays a role in neural development.
• Associated with neurodevelopmental disorders; mutations in EBF3 have been linked to hypotonia, ataxia, and delayed development syndrome (HADDS).
• Contains zinc-finger motifs and is a metal-binding protein involved in DNA-binding and transcriptional regulation.
• Undergoes alternative splicing, yielding multiple isoforms with potentially distinct functions.

Experimental Guidance and Technical Tips

• The immunogen was designed against a region within amino acids 400-500 of human EBF3, which may confer specific recognition of the target protein in Western blot and ELISA applications.
• For Western blot, consider a dilution range suitable for polyclonal antibodies and validate in your relevant sample system; the observed protein weight is approximately 65 kDa.
• Cross-reactivity with rat has been observed, making this antibody potentially suitable for studies in rat models; however, validation under your experimental conditions is recommended.
• As a polyclonal antibody, it may recognize multiple epitopes, which can enhance sensitivity but may also require careful titration to minimize background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop sourcing platform for IVD raw materials, encompassing validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-EBF3 polyclonal antibody supports research into transcriptional regulation and neurodevelopmental disorders, complementing our portfolio of rare target reagents. Partner with CamelBio to streamline your supply chain from concept to clinic.