Anti-EBAG9 Rabbit Polyclonal Antibody for WB, ELISA - O00559

Core Product Specifications and Parameters

Biological Background: EBAG9 Function and Localization

• EBAG9 (Estrogen receptor-binding fragment-associated gene 9), also known as RCAS1 or PDAF, is a receptor-binding cancer antigen originally identified on SiSo cells.
• The protein is predicted to contain a signal-anchor transmembrane domain and localizes to the Golgi apparatus membrane.
• Functionally, EBAG9 may participate in the suppression of cell proliferation and induce apoptotic cell death via activation of interleukin-1-beta converting enzyme (ICE)-like proteases.
• It is widely expressed at the mRNA level in tissues such as ovary, testis, prostate, thymus, muscle, and heart, but the protein is not detected in any of these organs, suggesting post-transcriptional regulation.
• The gene undergoes alternative splicing, producing isoforms that may have distinct functions.
• EBAG9 is a phosphoprotein and contains coiled-coil regions, motifs often involved in protein-protein interactions.

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weight is 24 kDa; consider using appropriate gel percentage and verify with positive control lysates from human or mouse tissues known to express EBAG9 mRNA, though protein detection may require optimization.
• For ELISA, the antibody can be used for detection of recombinant or native EBAG9; validate in the relevant sample system.
• Since the antibody is polyclonal and may recognize multiple epitopes, consider optimizing blocking and washing conditions to reduce non-specific binding.
• The immunogen covers amino acids 28–213, excluding the transmembrane region; thus the antibody likely targets the ectodomain and may recognize soluble forms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with integrated access to IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies like this EBAG9 Rabbit pAb for apoptosis and cancer studies, and bulk ancillary reagents. As your one-stop sourcing bridge, we support your entire workflow from concept to clinic, including sourcing for rare targets such as RCAS1.