Anti-EARS2 Rabbit Polyclonal Antibody for WB, ELISA - Q5JPH6

Core Product Specifications and Parameters

Biological Background: EARS2 Function and Localization

• EARS2 encodes the mitochondrial nondiscriminating glutamyl-tRNA synthetase (mtGluRS), which uniquely aminoacylates both tRNA(Glu) and tRNA(Gln) with glutamate, a critical step for mitochondrial protein translation. Related references: PMID:19805282
• The enzyme operates via a two-step ATP-dependent mechanism: glutamate is first activated to Glu-AMP, then transferred to the 3' end of the cognate tRNAs. Related references: PMID:19805282
• EARS2 belongs to the class-I aminoacyl-tRNA synthetase family and contains conserved domains for ATP binding, RNA binding, and ligase activity.
• The protein localizes to the mitochondrial matrix, directed by an N-terminal transit peptide.
• Alternative splicing generates multiple transcript variants, potentially regulating enzymatic function or mitochondrial targeting.
• Post-translational acetylation has been identified and may modulate activity or stability.
• Mutations in EARS2 are associated with primary mitochondrial disease, including combined oxidative phosphorylation deficiency 12 (COXPD12).

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 42–220, covering a conserved region likely exposed in native and denatured conditions, making the antibody suitable for WB and ELISA.
• For Western blot, consider loading 20–30 µg of mitochondrial-enriched or whole-cell lysate; expect a band near 59 kDa. Validate in your sample system, as post-translational modifications may cause slight shifts.
• The antibody cross-reacts with mouse EARS2; when using mouse tissues or cells, verify specificity with appropriate controls (e.g., knockdown or knockout models).
• For ELISA, optimize coating antigen (recombinant protein or peptide) and antibody dilution; a direct ELISA format can be used to assess antibody titer and binding affinity.
• Store aliquots at -20°C or -80°C. Avoid repeated freeze-thaw cycles to maintain activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This EARS2 antibody addresses mitochondrial biology and aminoacyl-tRNA synthetase research demands, available alongside validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and custom rare-target sourcing to streamline your development pipeline.