Anti-EAF1 Polyclonal Antibody for WB, ELISA - Q96JC9

Core Product Specifications and Parameters

Biological Background: EAF1 Function and Localization

• EAF1 (ELL-associated factor 1) is a transcriptional activator that enhances the elongation activities of ELL and ELL2, key regulators of RNA polymerase II transcription elongation.
• It is primarily localized within the nucleus, concentrating in nuclear speckles, which are enriched in pre-mRNA splicing factors, and Cajal bodies, involved in snRNP biogenesis.
• EAF1 exhibits broad tissue expression, with strong detection in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, prostate, testis, small intestine, and colon; expression is low in the thymus.
• The protein is classified as a phosphoprotein, suggesting that its activity or localization may be modulated by phosphorylation events.
• Alternative splicing of the EAF1 gene generates multiple transcript variants, potentially producing isoforms with distinct functional properties.
• As a component of the reference proteome, EAF1 is well-conserved and serves as a model for studying transcription regulation and elongation control.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 50–120 of human EAF1; consider validating specificity using cells with EAF1 knockout or knockdown to confirm band identity in Western blot.
• For Western blot, a primary band is expected at approximately 29 kDa; however, alternative splicing may yield additional isoforms—validate in your specific tissue or cell lysate.
• ELISA performance depends on optimal coating conditions and antibody–antigen pairing; careful optimization of dilutions and blocking steps is advised.
• Reported cross-reactivity includes human and rat; if testing other species, perform a sequence alignment of the immunogen region and conduct pilot experiments.
• Usage beyond WB and ELISA (e.g., IHC, IF) has not been validated; thorough optimization is required before applying to other techniques.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw materials, bridging the gap from concept to clinical application. For transcription regulation research, including EAF1-related targets, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Whether you need rare-target sourcing or scalable production, our one-stop platform accelerates your assay development and manufacturing timelines.